Evaluation of a simple in-house test to presumptively differentiate Mycobacterium tuberculosis complex from nontuberculous mycobacteria by detection of p-nitrobenzoic acid metabolites.
Evaluation of a simple in-house test to presumptively differentiate Mycobacterium tuberculosis complex from nontuberculous mycobacteria by detection of p-nitrobenzoic acid metabolites.
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通过检测对硝基苯甲酸代谢物推定区分结核分枝杆菌复合体和非结核分枝杆菌的简单内部测试的评估
DOI：
10.1371/journal.pone.0080877
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Huang H
中科院分区：
文献类型：
--
作者：
Wang G;Yu X;Liang Q;Chen S;Wilson S;Huang H
在患者护理中，迫切需要及时区分结核分枝杆菌复合群（MTC）和非结核分枝杆菌（NTM）种类，因为常规实验室方法耗时且繁琐。一种能够成功区分MTC和NTM的简便且廉价的方法得以建立并评估。本研究纳入了38株分枝杆菌模式菌株和参考菌株以及代表10种分枝杆菌的65株临床分离株。采用液相色谱 - 串联质谱法（LC/MS/MS）鉴定对硝基苯甲酸（PNB）还原的代谢产物。开发了一种分光光度法来检测这些代谢产物，并在多种MTC和NTM种类上进行了评估。所有受试的NTM种类和菌株都能将PNB还原为对氨基苯甲酸（PABA），而MTC菌株均未显示出类似活性。在受试的模式菌株和临床分离株中，对PABA的分光光度检测在MTC和NTM区分上具有100%的敏感性和特异性。PABA被确定为PNB还原的代谢产物之一。所有受试的NTM种类都将PNB代谢为PABA，而MTC成员缺乏这种活性。基于PABA产生建立了一种简单、特异且具有成本效益的方法，以便从培养的微生物中区分MTC和NTM。
The timely differentiation of Mycobacterium tuberculosis complex (MTC) and non-tubercular mycobacterium (NTM) species is urgently needed in patient care since the routine laboratory method is time consuming and cumbersome. An easy and cheap method which can successfully distinguish MTC from NTM was established and evaluated. 38 mycobacterial type and reference strains and 65 clinical isolates representing 10 species of mycobacterium were included in this study. Metabolites of p-nitrobenzoic acid (PNB) reduction were identified using liquid chromatography and tandem mass spectrometry (LC/MS/MS). A spectrophotometric method was developed to detect these metabolites, which was evaluated on a number of MTC and NTM species. All of the tested NTM species and strains reduced PNB to p-aminobenzoic acid (PABA), while none of the MTC strains showed a similar activity. Spectrophotometric detection of PABA had 100% sensitivity and specificity for MTC and NTM differentiation among the type strains and the clinical isolates tested. PABA was identified as one of the metabolites of PNB reduction. All the tested NTM species metabolized PNB to PABA whereas the MTC members lacked this activity. A simple, specific and cost-effective method based on PABA production was established in order to discriminate MTC from NTM from cultured organisms.
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影响因子：
4.4
作者：
RAFII, F;FRANKLIN, W;CERNIGLIA, CE
通讯作者：
CERNIGLIA, CE
影响因子：
1.6
作者：
Rodrigues, C.;Shenai, S.;Mehta, A.
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影响因子：
56.9
作者：
ANSBACHER, S.
通讯作者：
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5.9
作者：
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通讯作者：
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影响因子：
9.4
作者：
GROSS, WM;HAWKINS, JE
通讯作者：
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