Nestin expression correlates with nerve and retroperitoneal tissue invasion in pancreatic cancer.
Nestin expression correlates with nerve and retroperitoneal tissue invasion in pancreatic cancer.
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DOI：
10.1016/j.humpath.2008.02.022
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发表时间：
2009-02
期刊：
影响因子：
3.3
通讯作者：
Tajiri, Takashi
中科院分区：
文献类型：
--
作者：
Kawamoto, Masao;Ishiwata, Toshiyuki;Cho, Kazumitsu;Uchida, Eiji;Korc, Murray;Naito, Zenya;Tajiri, Takashi
Nestin首先被描述为在神经上皮干细胞中表达的中间丝蛋白。巢蛋白的表达也被报道在脑肿瘤、神经鞘瘤、胃肠道间质瘤和黑色素瘤中。在胰腺中，巢蛋白的表达已被检测到外分泌和间充质细胞，包括星状细胞，周细胞和内皮细胞。在本研究中，我们研究了巢蛋白在人胰腺导管腺癌中的表达，并试图确定其在这种恶性肿瘤中的作用。RT-PCR检测结果显示，所有10种胰腺癌细胞株中均有Nestin mRNA的表达，定量RT-PCR检测结果显示，PANC-1细胞中Nestin mRNA的表达量最高，PK-8细胞中最低。免疫荧光分析显示Nestin定位于PANC-1细胞的胞浆外侧。60例癌组织中20例（33.3%）癌细胞巢蛋白阳性，原位杂交证实其表达。Nestin在癌细胞周围神经纤维和癌细胞浸润周围神经纤维中的表达也增加。在临床病理学上，胰腺癌细胞中Nestin的表达与神经浸润（p=0.010）以及肿瘤切除边缘中癌细胞的存在（p=0.003）之间存在统计学显著相关性。Nestin阳性病例与Nestin阴性病例相比，无论是否给予辅助治疗，切除后的生存率相似。这些结果表明，巢蛋白表达在胰腺癌细胞可能有助于神经和间质浸润在这种恶性肿瘤。
Nestin was first described as an intermediate filament protein expressed in neuroepithelial stem cells. Nestin expression has also been reported in brain tumors, schwannomas, gastrointestinal stromal tumors, and melanomas. In the pancreas, Nestin expression has been detected in exocrine and mesenchymal cells, including stellate cells, pericytes, and endothelial cells. In the present study, we examined Nestin expression in human pancreatic ductal adenocarcinoma and sought to determine its role in this malignancy. RT-PCR analysis demonstrated the presence of Nestin mRNA in all ten tested pancreatic cancer cell lines, and quantitative RT-PCR revealed that Nestin mRNA levels were highest in PANC-1 cells and lowest in PK-8 cells. Immunofluorescent analysis revealed that Nestin localized in the outer cytoplasm of PANC-1 cells. Nestin immunoreactivity was present in the cancer cells in 20 of 60 (33.3 %) cancer cases, and its expression was confirmed by in situ hybridization. Nestin expression was also increased in peripheral nerve fibers adjacent to cancer cells and in peripheral nerve fibers invaded by cancer cells. Clinicopathologically, there was a statistically significant association between Nestin expression in pancreatic cancer cells and nerve invasion (p=0.010), and the presence of cancer cells in the tumor resection margins (p=0.003). Nestin-positive cases exhibited similar survival following resection by comparison with Nestin-negative cases, irrespective of whether or not they were given adjuvant therapy. These findings indicate that Nestin expression in pancreatic cancer cells may contribute to nerve and stromal invasion in this malignancy.
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影响因子：
6.1
作者：
LIN, RCS;MATESIC, DF;BRUSTLE, O
通讯作者：
BRUSTLE, O
影响因子：
2.5
作者：
Schiffer, Davide;Manazza, Andrea;Tamagno, Ilaria
通讯作者：
Tamagno, Ilaria
影响因子：
2.2
作者：
Shimada, Satoko;Tsuzuki, Toyonori;Illei, Peter B.
通讯作者：
Illei, Peter B.
影响因子：
11.2
作者：
Kleeberger, Wolfram;Bova, G. Steven;Berman, David M.
通讯作者：
Berman, David M.
DOI：
10.1073/pnas.0701117104
发表时间：
2007-03-13
影响因子：
11.1
作者：
Carriere, Catherine;Seeley, Elliott S.;Korc, Murray
通讯作者：
Korc, Murray