In vitro evaluation of carbon-nanotube-reinforced bioprintable vascular conduits.
In vitro evaluation of carbon-nanotube-reinforced bioprintable vascular conduits.
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DOI：
10.1088/0957-4484/25/14/145101
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发表时间：
2014-04-11
期刊：
影响因子：
3.5
通讯作者：
Ozbolat IT
中科院分区：
文献类型：
--
作者：
Dolati F;Yu Y;Zhang Y;De Jesus AM;Sander EA;Ozbolat IT
厚的工程化组织和器官结构如心脏、肝脏、胰腺或肾脏的血管化仍然是组织工程中的主要挑战。血管化需要通过具有高灌注能力和显著的机械强度和弹性的网络来供应氧气和营养并清除活组织和器官中的废物。在本文中，我们介绍了一种直接打印血管导管的制造工艺，其中导管用碳纳米管（CNT）增强，以提高其机械性能和生物可打印性。对封装在人冠状动脉平滑肌细胞（HCASMC）中的打印导管进行体外评价，以表征CNT增强对导管的机械、灌注和生物学性能的影响。灌注和渗透性，细胞活力，细胞外基质形成和组织组织学进行了评估和讨论，得出的结论是，CNT增强的血管导管提供了一个基础，机械吸引力的结构，其中CNT可以取代天然蛋白质纳米纤维，进一步整合这些导管在大规模组织制造。
Vascularization of thick engineered tissue and organ constructs like the heart, liver, pancreas or kidney remains a major challenge in tissue engineering. Vascularization is needed to supply oxygen and nutrients and remove waste in living tissues and organs through a network that should possess high perfusion ability and significant mechanical strength and elasticity. In this paper, we introduce a fabrication process to print vascular conduits directly, where conduits were reinforced with carbon-nanotubes (CNTs) to enhance their mechanical properties and bioprintability. In vitro evaluation of printed conduits encapsulated in human coronary artery smooth muscle cells (HCASMCs) was performed to characterize the effects of CNT reinforcement on the mechanical, perfusion and biological performance of the conduits. Perfusion and permeability, cell viability, extracellular matrix formation and tissue histology were assessed and discussed, and it was concluded that CNT-reinforced vascular conduits provided a foundation for mechanically appealing constructs where CNTs could be replaced with natural protein nanofibers for further integration of these conduits in large-scale tissue fabrication.
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