Cellular interactions with hydrogel microfibers synthesized via interfacial tetrazine ligation.
Cellular interactions with hydrogel microfibers synthesized via interfacial tetrazine ligation.
复制标题
DOI：
10.1016/j.biomaterials.2018.06.042
复制
发表时间：
2018-10
期刊：
影响因子：
14
通讯作者：
Jia X
中科院分区：
文献类型：
--
作者：
Liu S;Moore AC;Zerdoum AB;Zhang H;Scinto SL;Zhang H;Gong L;Burris DL;Rajasekaran AK;Fox JM;Jia X
天然细胞外基质（ECM）中发现的纤维蛋白在伤口愈合和组织修复过程中充当内源性细胞迁移和生长的宿主基质。尽管已经开发了各种纤维支架来概括天然ECM的微观结构，但是机械上坚固且具有生物活性的水凝胶微纤维的容易合成一直是难以捉摸的。本文描述了使用界面生物正交聚合通过四嗪连接产生基于水凝胶的微纤维支架。在油-水界面处的三官能应变反式环辛烯单体和双官能S-四嗪单体的组合导致在细胞培养条件下稳定的微纤维的形成。合成的生物正交性质允许将四嗪缀合的肽或蛋白质与位点选择性地遗传编码的四嗪直接掺入。微纤维提供物理引导和生化信号以促进成纤维细胞的附着、分裂和迁移。机制研究表明，纤维引导的细胞迁移是F-肌动蛋白和微管依赖，确认接触指导的微纤维。在存在分离的微纤维的情况下延长成纤维细胞的培养导致形成包裹在纤维芯周围的多层细胞片。纤维网提供3D模板以促进细胞浸润和组织样生长。总的来说，生物正交方法导致直接合成交联水凝胶微纤维，其可潜在地用作组织修复和再生的指导材料。
Fibrous proteins found in the natural extracellular matrix (ECM) function as host substrates for migration and growth of endogenous cells during wound healing and tissue repair processes. Although various fibrous scaffolds have been developed to recapitulate the microstructures of the native ECM, facile synthesis of hydrogel microfibers that are mechanically robust and biologically active have been elusive. Described herein is the use of interfacial bioorthogonal polymerization to create hydrogel-based microfibrous scaffolds via tetrazine ligation. Combination of a trifunctional strained trans-cyclooctene monomer and a difunctional s-tetrazine monomer at the oil-water interface led to the formation of microfibers that were stable under cell culture conditions. The bioorthogonal nature of the synthesis allows for direct incorporation of tetrazine-conjugated peptides or proteins with site-selectively, genetically encoded tetrazines. The microfibers provide physical guidance and biochemical signals to promote the attachment, division and migration of fibroblasts. Mechanistic investigations revealed that fiber-guided cell migration was both F-actin and microtubule-dependent, confirming contact guidance by the microfibers. Prolonged culture of fibroblasts in the presence of an isolated microfiber resulted in the formation of a multilayered cell sheet wrapping around the fiber core. A fibrous mesh provided a 3D template to promote cell infiltration and tissue-like growth. Overall, the bioorthogonal approach led to the straightforward synthesis of crosslinked hydrogel microfibers that can potentially be used as instructive materials for tissue repair and regeneration.
登录
查看更多内容
DOI：
10.1039/c4cc09568e
发表时间：
2015-03-28
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Liu S;Dicker KT;Jia X
通讯作者：
Jia X
影响因子：
15
作者：
Hansell, Claire F.;Espeel, Pieter;O'Reilly, Rachel K.
通讯作者：
O'Reilly, Rachel K.
影响因子：
4.1
作者：
Browne, Shane;Zeugolis, Dimitrios I.;Pandit, Abhay
通讯作者：
Pandit, Abhay
影响因子：
29.4
作者：
Liu, Shuang;Zhang, Han;Remy, Roddel A.;Deng, Fei;Mackay, Michael E.;Fox, Joseph M.;Jia, Xinqiao
通讯作者：
Jia, Xinqiao
影响因子：
11.2
作者：
Jing, Xin;Mi, Hao-Yang;Turng, Lih-Sheng
通讯作者：
Turng, Lih-Sheng