Expression of proliferin-related protein in testis and the biological significance in testosterone production
Expression of proliferin-related protein in testis and the biological significance in testosterone production
复制标题
睾丸中增殖素相关蛋白的表达及其在睾酮产生中的生物学意义
DOI：
10.1016/j.mce.2011.05.046
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发表时间：
2011-08
影响因子：
4.1
通讯作者：
Jie Hao
中科院分区：
文献类型：
--
作者：
Lina Zhao;Jie Hao
增殖蛋白相关蛋白（PRP）最初被鉴定为小鼠胎盘中的血管生成抑制剂。事实上，PRP 的组织表达主要在胎盘中被记录。我们在此首次报道 PRP 在雄性大鼠睾丸中表达。免疫细胞化学和原位杂交结果显示 Leydig 细胞中 PRP 免疫染色呈阳性。 TM3 Leydig 细胞系中 PRP 的免疫荧光染色表明 PRP 位于细胞质内。大鼠睾丸中 PRP 的表达模式表现出与年龄相关的增加。 HCG通过PKA途径显着上调TM3细胞中PRP的表达水平。为了阐明 PRP 的功能，进行了实验来检查 PRP 的慢病毒介导的 RNA 干扰 (RNAi) 对睾酮产生和涉及类固醇生成的几个基因表达的影响。 PRP 沉默导致 HCG 刺激的睾酮产生减少。此外，PRP 沉默减弱了 HCG 刺激后 PRLR mRNA 的增加。此外，PRP 沉默后，PRL 对 HCG 诱导的睾酮产生的增强作用也减弱，表明 PRP 可能通过影响响应刺激的 PRL 受体表达来参与 PRL 功能。总而言之，这些数据表明 PRP 受 HCG 调节，并在男性生殖中发挥作用，例如睾酮的产生。
Proliferin-related protein (PRP) was originally identified as an angiogenesis inhibitor in mouse placentas. Indeed, the tissue expression of PRP has mainly been documented in placentas. We report herein for the first time that PRP is expressed in male rat testes. Immunocytochemical and in situ hybridization results showed positive PRP immunostaining in Leydig cells. Immunofluorescent staining of PRP in the TM3 Leydig cell line indicates that PRP is located within the cytoplasm. The expression pattern of PRP in rat testis exhibited an age-related increase. HCG significantly up-regulated the level of expression of PRP in TM3 cells via the PKA pathway. To elucidate the function of PRP, experiments were conducted to examine the consequences of lentiviral-mediated RNA interference (RNAi) of PRP on testosterone production and expression of several genes involved in steroidogenesis. PRP silencing caused a decrease in HCG-stimulated testosterone production. In addition, PRP silencing attenuated the increase in PRLR mRNA following HCG stimulation. Moreover, the enhanced effect of PRL on HCG-induced testosterone production was also weakened following PRP silencing, indicating that PRP may be involved in PRL function through an effect on PRL receptor expression in response to stimuli. Taken together, these data suggest that PRP is regulated by HCG and plays roles in male reproduction, such as testosterone production.
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DOI：
10.1095/biolreprod63.2.519
发表时间：
2000-08
期刊：
--
影响因子：
--
作者：
D. Toft;D. Linzer
通讯作者：
D. Toft;D. Linzer
影响因子：
--
作者：
R. Maran;J. Arunakaran;M. M. Aruldhas-M.
通讯作者：
R. Maran;J. Arunakaran;M. M. Aruldhas-M.
影响因子：
4.1
作者：
N. Rahman;I. Huhtaniemi
通讯作者：
N. Rahman;I. Huhtaniemi
影响因子：
5.8
作者：
Travison, Thomas G.;Morley, John E.;McKinlay, John B.
通讯作者：
McKinlay, John B.
影响因子：
4.7
作者：
Marian Leimbach;O. Edenhofer
通讯作者：
Marian Leimbach;O. Edenhofer