Terminal field specificity of forebrain efferent axons to brainstem gustatory nuclei.
Terminal field specificity of forebrain efferent axons to brainstem gustatory nuclei.
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DOI：
10.1016/j.brainres.2008.10.075
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发表时间：
2009-01-12
期刊：
影响因子：
2.9
通讯作者：
Lundy RF
中科院分区：
文献类型：
--
作者：
Kang Y;Lundy RF
吻侧前脑结构如味觉皮层（GC）、终纹床核（BNST）、杏仁中央核（CeA）和下丘脑外侧核（LH）向孤束核（NST）和臂旁核（PBN）发送投射，调节味觉引起的反应。然而，前脑诱导的兴奋性和抑制性作用的比例往往不同时，味觉细胞记录从NST的PBN的变化。本研究调查了这种下降的影响是否源于前脑神经元的共享或不同的人口。在电生理引导下，将逆行示踪剂固蓝（FB）和荧光金（FG）或绿色（GFB）和红色（RFB）荧光乳胶微球经离子电渗或压力脉冲（10 ms，20 psi）注入6只大鼠的NST和同侧PBN的味觉反应区。7天后，对动物实施安乐死，并对含有LH、CeA、BNST和GC的组织切片进行处理，用于FB和FG或GFB和RFB的共定位。结果表明，CeA是NST（82.3±7.6个细胞/切片）和PBN（76.7±11.5个细胞/切片）的主要输入来源，而BNST（31.8±4.5; 37.0±4.8）、LH（35.0±5.4; 33.6±5.7）和GC（27.5±4.0; 29.0±4.6）则是主要输入来源。在逆行标记的细胞总数中，示踪剂共定位的发生率在GC中为17±3%，在CeA中为17±2%，在BNST中为15±3%，在LH中为16±1%。因此，无论前脑来源的味觉NST和PBN的大部分下行输入来自不同的神经元群体。这种布置为上行味觉系统的第一和第二中枢中继中的味觉处理的差异调节提供了解剖学基础。
Rostral forebrain structures like the gustatory cortex (GC), bed nucleus of the stria terminalis (BNST), central nucleus of the amygdala (CeA), and lateral hypothalamus (LH) send projections to the nucleus of solitary tract (NST) and the parabrachial nucleus (PBN) that modulate taste-elicited responses. However, the proportion of forebrain-induced excitatory and inhibitory effects often differs when taste cell recording changes from the NST to the PBN. The present study investigated whether this descending influence originates from a shared or distinct population of forebrain neurons. Under electrophysiological guidance, the retrograde tracers fast blue (FB) and fluorogold (FG) or green (GFB) and red (RFB) fluorescent latex microbeads were injected iontophoretically or by pressure pulses (10 ms at 20 psi) into the taste-responsive regions of the NST and the ipsilateral PBN in six rats. Seven days later, the animals were euthanized and tissue sections containing the LH, CeA, BNST, and GC were processed for co-localization of FB and FG or GFB and RFB. The results showed that the CeA is the major source of input to the NST (82.3±7.6 cells/section) and the PBN (76.7±11.5), compared to the BNST (31.8±4.5; 37.0±4.8), the LH (35.0±5.4; 33.6±5.7), and the GC (27.5±4.0; 29.0±4.6). Of the total number of retrogradely labeled cells, the incidence of tracer co-localization was 17±3% in the GC, 17±2% in the CeA, 15±3% in the BNST and 16±1% in the LH. Thus, irrespective of forebrain source the majority of descending input to the gustatory NST and PBN originates from distinct neuronal populations. This arrangement provides an anatomical substrate for differential modulation of taste processing in the first and second central relays of the ascending gustatory system.
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