Distinct roles of TRP channels in auditory transduction and amplification in Drosophila.
Distinct roles of TRP channels in auditory transduction and amplification in Drosophila.
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DOI：
10.1016/j.neuron.2012.11.030
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发表时间：
2013-01-09
期刊：
影响因子：
16.2
通讯作者：
Wilson RI
中科院分区：
文献类型：
--
作者：
Lehnert BP;Baker AE;Gaudry Q;Chiang AS;Wilson RI
听觉受体细胞依靠机械门控通道将声音刺激转化为神经活动。几个TRP通道与果蝇的听觉转导有关，但由于无法记录来自受体神经元的阈下信号，机制研究受到阻碍。在这里，我们开发了一种非侵入性的方法来测量这些信号，通过记录来自中央神经元的电偶联到一个遗传上定义的听觉受体细胞群。我们发现TRPN家族成员NompC是听觉器官主动放大声诱发运动所必需的，但在听觉受体细胞中并不需要转导。相反，NompC使转导复合物对运动敏感，并精确调节复合物上的静力。相比之下，TRPV通道Nanchung和Inactive是声音响应所必需的，这表明它们是转导复合物的组成部分。因此，在果蝇听力中，转导和主动放大是基因上可分离的过程。
Auditory receptor cells rely on mechanically gated channels to transform sound stimuli into neural activity. Several TRP channels have been implicated in Drosophila auditory transduction, but mechanistic studies have been hampered by the inability to record subthreshold signals from receptor neurons. Here, we develop a non-invasive method for measuring these signals by recording from a central neuron that is electrically coupled to a genetically defined population of auditory receptor cells. We find that the TRPN family member NompC, which is necessary for the active amplification of sound-evoked motion by the auditory organ, is not required for transduction in auditory receptor cells. Instead, NompC sensitizes the transduction complex to movement and precisely regulates the static forces on the complex. In contrast, the TRPV channels Nanchung and Inactive are required for responses to sound, suggesting they are components of the transduction complex. Thus, transduction and active amplification are genetically separable processes in Drosophila hearing.
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