Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia.
Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia.
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DOI：
10.1371/journal.pone.0011012
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发表时间：
2010-06-08
期刊：
影响因子：
3.7
通讯作者：
Chung YD
中科院分区：
文献类型：
--
作者：
Lee J;Moon S;Cha Y;Chung YD
TRPN通道蛋白对昆虫机械感觉神经元和脊椎动物毛细胞的感觉传导至关重要。果蝇TRPN同源物NOMPC在触觉刷毛中产生机械受体电位和电流是必需的。果蝇触角耳的弦状神经元的转导也需要NOMPC和TRPV通道，但TRPN或TRPV通道在转导和调节主动触角力学方面具有不同的作用。有证据表明NOMPC是一个主要的机械换能器通道，但其亚细胞位置（了解其在转导中的确切作用的关键）尚未确定。在这里，通过免疫染色，我们将NOMPC定位在外部和chordotonal感觉神经元的机械感觉纤毛的尖端，这与预测的机械传感器通道一致。在chordoonal神经元中，TRPN和TRPV通道分别分离到远端和近端纤毛区。这种带状分离是由纤毛扩张划定的，纤毛扩张是纤毛内束内运输（IFT）蛋白的纤毛内组装。我们的研究结果为NOMPC作为果蝇感觉器官的主要转导通道提供了强有力的证据。这些数据还揭示了听觉弦索转导模型的结构基础，其中TRPN和TRPV通道在受体电位的产生和放大中依次发挥作用，但在调节主动纤毛运动中发挥相反的作用。
A TRPN channel protein is essential for sensory transduction in insect mechanosensory neurons and in vertebrate hair cells. The Drosophila TRPN homolog, NOMPC, is required to generate mechanoreceptor potentials and currents in tactile bristles. NOMPC is also required, together with a TRPV channel, for transduction by chordotonal neurons of the fly's antennal ear, but the TRPN or TRPV channels have distinct roles in transduction and in regulating active antennal mechanics. The evidence suggests that NOMPC is a primary mechanotransducer channel, but its subcellular location—key for understanding its exact role in transduction—has not yet been established. Here, by immunostaining, we locate NOMPC at the tips of mechanosensory cilia in both external and chordotonal sensory neurons, as predicted for a mechanotransducer channel. In chordotonal neurons, the TRPN and TRPV channels are respectively segregated into distal and proximal ciliary zones. This zonal separation is demarcated by and requires the ciliary dilation, an intraciliary assembly of intraflagellar transport (IFT) proteins. Our results provide a strong evidence for NOMPC as a primary transduction channel in Drosophila mechansensory organs. The data also reveals a structural basis for the model of auditory chordotonal transduction in which the TRPN and TRPV channels play sequential roles in generating and amplifying the receptor potential, but have opposing roles in regulating active ciliary motility.
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影响因子：
64.8
作者：
Yorozu, Suzuko;Wong, Allan;Fischer, Brian J.;Dankert, Heiko;Kernan, Maurice J.;Kamikouchi, Azusa;Ito, Kei;Anderson, David J.
通讯作者：
Anderson, David J.
影响因子：
64.8
作者：
Zelhof, Andrew C.;Hardy, Robert W.;Zuker, Charles S.
通讯作者：
Zuker, Charles S.
DOI：
10.1016/j.cub.2008.11.020
发表时间：
2008-12-23
期刊：
Current biology : CB
影响因子：
--
作者：
Lee E;Sivan-Loukianova E;Eberl DF;Kernan MJ
通讯作者：
Kernan MJ
影响因子：
56.9
作者：
Sidi, S;Friedrich, RW;Nicolson, T
通讯作者：
Nicolson, T
影响因子：
5.3
作者：
Gong, ZF;Son, WS;Kim, CS
通讯作者：
Kim, CS