SARS-CoV-2 S1 and N-based serological assays reveal rapid seroconversion and induction of specific antibody response in COVID-19 patients.
SARS-CoV-2 S1 and N-based serological assays reveal rapid seroconversion and induction of specific antibody response in COVID-19 patients.
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DOI：
10.1038/s41598-020-73491-5
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发表时间：
2020-10-06
影响因子：
4.6
通讯作者：
Hashem AM
中科院分区：
文献类型：
--
作者：
Algaissi A;Alfaleh MA;Hala S;Abujamel TS;Alamri SS;Almahboub SA;Alluhaybi KA;Hobani HI;Alsulaiman RM;AlHarbi RH;ElAssouli MA;Alhabbab RY;AlSaieedi AA;Abdulaal WH;Al-Somali AA;Alofi FS;Khogeer AA;Alkayyal AA;Mahmoud AB;Almontashiri NAM;Pain A;Hashem AM
由于由新型SARS-CoV-2引起的2019冠状病毒病（COVID-19）继续在全球迅速传播，需要经过充分验证的血清学分析，以便检测COVID-19患者或康复个体的病毒特异性抗体反应。在本研究中，我们建立并使用基于间接酶联免疫吸附试验（ELISA）的多种血清学检测方法来研究COVID-19患者的抗体反应。为了验证这些检测方法，我们使用大流行前健康对照、发病后不同时间点的COVID-19患者和其他人类冠状病毒（cov）血清阳性血清来确定检测方法的截断值、敏感性和特异性。构建的刺突糖蛋白-核衣壳(N)酶联免疫吸附检测试剂盒不仅具有较高的特异性和敏感性，而且与其他冠状病毒无交叉反应性。我们还发现，在我们的研究中检测的所有RT-PCR确诊的COVID-19患者早在发病后第一周就产生了病毒特异性IgM和IgG抗体。我们的数据还表明，在血清学检测中同时纳入S1和N比单独使用其中任何一种都能捕获尽可能多的潜在SARS-CoV-2阳性病例。这对于追踪接触者和病例以及开展大规模流行病学研究以了解病毒在人群中传播的真实程度尤其重要。
As the Coronavirus Disease 2019 (COVID-19), which is caused by the novel SARS-CoV-2, continues to spread rapidly around the world, there is a need for well validated serological assays that allow the detection of viral specific antibody responses in COVID-19 patients or recovered individuals. In this study, we established and used multiple indirect Enzyme Linked Immunosorbent Assay (ELISA)-based serological assays to study the antibody response in COVID-19 patients. In order to validate the assays we determined the cut off values, sensitivity and specificity of the assays using sera collected from pre-pandemic healthy controls, COVID-19 patients at different time points after disease-onset, and seropositive sera to other human coronaviruses (CoVs). The developed SARS-CoV-2 S1 subunit of the spike glycoprotein and nucleocapsid (N)-based ELISAs not only showed high specificity and sensitivity but also did not show any cross-reactivity with other CoVs. We also show that all RT-PCR confirmed COVID-19 patients tested in our study developed both virus specific IgM and IgG antibodies as early as week one after disease onset. Our data also suggest that the inclusion of both S1 and N in serological testing would capture as many potential SARS-CoV-2 positive cases as possible than using any of them alone. This is specifically important for tracing contacts and cases and conducting large-scale epidemiological studies to understand the true extent of virus spread in populations.
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