Antigenic variation among bovine enteric coronaviruses (BECV) and bovine respiratory coronaviruses (BRCV) detected using monoclonal antibodies.
Antigenic variation among bovine enteric coronaviruses (BECV) and bovine respiratory coronaviruses (BRCV) detected using monoclonal antibodies.
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DOI：
10.1007/s007050050656
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发表时间：
1999
影响因子：
2.7
通讯作者：
Saif LJ
中科院分区：
文献类型：
--
作者：
Hasoksuz M;Lathrop S;Al-dubaib MA;Lewis P;Saif LJ
牛冠状病毒可引起成年奶牛新生小牛腹泻(CD)，并与冬季痢疾(WD)有关。它也可以从进入饲养场的牛的呼吸道中分离出来。用2株牛肠道冠状病毒(BECV)和2株牛呼吸道冠状病毒(BRCV)的血凝素酯酶(HE)和刺突(S)表面蛋白的单抗(MAb)分别与6株BECV和6株新近分离的BRCV进行抗原性试验，以研究不同组织嗜性的BCV株的抗原性。所有单抗对BECV和BRCV毒株均有较高的免疫荧光(IF)滴度，表明具有保守的交叉反应表位。在血凝抑制(HI)试验中，S单抗比HE单抗具有更广泛的反应性。在HI中，BRCV和CD单抗比WD单抗具有更广泛的反应性。梅毒疫苗CD株的血凝素活性不受任何单抗的抑制。在6个BRCV分离物中，BRCV株R6的HI活性是独一无二的。在病毒中和试验中，抗BRCV R4株的单抗中和了所有6个BECV株。BECV和BRCV毒株之间都存在抗原性变异，但不能完全归因于该毒株的临床来源。
Bovine coronavirus (BCV) causes neonatal calf diarrhea (CD) and is associated with winter dysentery (WD) in adult dairy cattle. It can also be isolated from the respiratory tracts of cattle entering feedlots. Monoclonal antibodies (MAbs) specific for the hemagglutinin esterase (HE) and spike (S) surface proteins of 2 bovine enteric coronavirus (BECV) strains and two bovine respiratory coronavirus (BRCV) strains were tested against 6 BECV strains and 6 recently isolated BRCV strains, in order to characterize the antigenicity of BCV strains with varied tissue tropisms. All MAbs had high immunofluorescence (IF) titers against BECV and BRCV strains, indicative of conserved cross-reactive epitopes. In hemagglutination inhibition (HI) tests, the S-MAbs were more broadly reactive than HE-MAbs. The BRCV and CD MAbs were more broadly reactive in HI than the WD MAbs. The HA activity of the Mebus vaccine CD strain was not inhibited by any of the MAbs tested. The HI activity of BRCV strain R6 was unique among the 6 BRCV isolates. In virus neutralization assays, MAbs to the BRCV strain R4 neutralized all 6 BECV strains tested. Antigenic variation exists among both BECV and BRCV strains, but it cannot be attributed soley to the clinical origin of the strain.
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影响因子：
3.3
作者：
MCNULTY, MS;BRYSON, DG;LOGAN, EF
通讯作者：
LOGAN, EF
影响因子：
1.2
作者：
TSUNEMITSU, H;YONEMICHI, H;SHIMIZU, M
通讯作者：
SHIMIZU, M
影响因子：
3.7
作者：
DEREGT, D;BABIUK, LA
通讯作者：
BABIUK, LA
影响因子：
9.4
作者：
Milane, G;Kourtesis, AB;Dea, S
通讯作者：
Dea, S
影响因子：
2.7
作者：
Welch SK;Saif LJ
通讯作者：
Saif LJ