Bile acid patterns in commercially available oxgall powders used for the evaluation of the bile tolerance ability of potential probiotics.
Bile acid patterns in commercially available oxgall powders used for the evaluation of the bile tolerance ability of potential probiotics.
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DOI：
10.1371/journal.pone.0192964
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Guo CF
中科院分区：
文献类型：
--
作者：
Hu PL;Yuan YH;Yue TL;Guo CF
本研究旨在分析市售牛胆粉中的胆汁酸模式，用于评价益生菌的胆汁耐受能力。将购自Sigma-Aldrich、Oxoid和BD Difco的Qxgall粉末溶解在蒸馏水中，并进行分析。结合胆汁酸进行了离子对高效液相色谱法（HPLC），游离胆汁酸检测其对溴苯甲酰甲基酯衍生物，用乙酸乙酯萃取后，使用反相HPLC，总胆汁酸进行了分析，通过酶比色法。结果显示，9种单独的胆汁酸（即，牛磺胆酸、甘氨胆酸、牛磺去氧胆酸、甘氨脱氧胆酸、牛磺鹅去氧胆酸、甘氨鹅去氧胆酸、胆酸、鹅去氧胆酸、脱氧胆酸）。3种牛胆粉的总胆汁酸含量相近，但各胆汁酸的相对含量差异极显著（P < 0.001）。来自Sigma-Aldrich的牛胆粉末在甘氨酸结合胆汁酸与牛磺酸结合胆汁酸、二羟基胆汁酸与三羟基胆汁酸以及游离胆汁酸与结合胆汁酸的比率方面比其他粉末更接近人胆汁。因此，可以使用Sigma-Aldrich的牛胆粉代替Oxoid和BD Difco的牛胆粉来评价益生菌作为人胆汁模型的胆汁耐受能力。
This study aimed to analyze the bile acid patterns in commercially available oxgall powders used for evaluation of the bile tolerance ability of probiotic bacteria. Qxgall powders purchased from Sigma-Aldrich, Oxoid and BD Difco were dissolved in distilled water, and analyzed. Conjugated bile acids were profiled by ion-pair high-performance liquid chromatography (HPLC), free bile acids were detected as their p-bromophenacyl ester derivatives using reversed-phase HPLC after extraction with acetic ether, and total bile acids were analyzed by enzymatic-colorimetric assay. The results showed that 9 individual bile acids (i.e., taurocholic acid, glycocholic acid, taurodeoxycholic acid, glycodeoxycholic acid, taurochenodeoxycholic acid, glycochenodeoxycholic acid, cholic acid, chenodeoxycholic acid, deoxycholic acid) were present in each of the oxgall powders tested. The content of total bile acid among the three oxgall powders was similar; however, the relative contents of the individual bile acids among these oxgall powders were significantly different (P < 0.001). The oxgall powder from Sigma-Aldrich was closer to human bile in the ratios of glycine-conjugated bile acids to taurine-conjugated bile acids, dihydroxy bile acids to trihydroxy bile acids, and free bile acids to conjugated bile acids than the other powders were. It was concluded that the oxgall powder from Sigma-Aldrich should be used instead of those from Oxoid and BD Difco to evaluate the bile tolerance ability of probiotic bacteria as human bile model.
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影响因子：
2.9
作者：
STAGGERS, JE;HERNELL, O;CAREY, MC
通讯作者：
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10.1016/j.ijfoodmicro.2011.05.003
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