Intensity distortion in dual-axis galvo mirror scanning TPF imaging system
Intensity distortion in dual-axis galvo mirror scanning TPF imaging system
复制标题
双轴振镜扫描TPF成像系统中的强度畸变
DOI：
10.1016/j.ijleo.2020.164311
复制
发表时间：
2020-03
期刊：
影响因子：
3.1
通讯作者：
Yuanqin Xia
中科院分区：
文献类型：
--
作者：
Guozhong Hou;Zhiwei Dong;Sheng Zhang;Zhibin Zhang;Yuanqin Xia
搭建了以双轴振镜为扫描单元的双光子荧光（TPF）成像系统。基于所搭建的成像系统光路，从理论上分析了双轴振镜扫描TPF成像系统产生强度畸变的机理。实验中，通过检测罗丹明B分子在宽带飞秒脉冲激发下产生的TPF信号，以罗丹明B样品实现TPF成像。成像结果验证了强度畸变的存在，其分布与理论分析吻合较好。为了消除TPF成像结果中的强度失真，提出了一种计算校准矩阵的数学方法，将校准矩阵应用到罗丹明B样品失真的成像结果中，有效地缓解了强度失真，获得了精确的成像结果。
Two-photon fluorescence (TPF) imaging system with dual-axis galvo mirror as scanning unit is built. Based on the optical path of imaging system built, mechanism of how intensity distortion generating in dual-axis galvo mirror scanning TPF imaging system is theoretically analyzed. In experiment, TPF imaging is realized with Rhodamine B sample through detecting TPF signal generated from Rhodamine B molecule at excitation of broadband femtosecond pulses. The imaging result verifies the existence of intensity distortion with distribution agreeing well with the theoretical analyzation. To eliminate the intensity distortion in TPF imaging result, a mathematical method is proposed in which a calibration matrix is calculated, As the calibration matrix is applied to the distorted imaging result of Rhodamine B sample, intensity distortion is relieved effectively and precise imaging result is achieved.
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