Got black swimming dots in your cell culture? Identification of Achromobacter as a novel cell culture contaminant.
Got black swimming dots in your cell culture? Identification of Achromobacter as a novel cell culture contaminant.
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DOI：
10.1016/j.biologicals.2009.09.006
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发表时间：
2010-03
期刊：
影响因子：
1.7
通讯作者：
Fenton, Jenifer Imig
中科院分区：
文献类型：
--
作者：
Gray, Jennifer Sue;Birmingham, Janette Marie;Fenton, Jenifer Imig
细胞培养模型系统因其易于使用、相对便宜和潜在的无限样本量而被利用。然而，当培养物含有污染时，无法获得可靠的结果。本报告讨论了在多个细胞系中观察到的移动黑斑的观察和鉴定。培养污染菌，从分离的菌落中纯化DNA。采用引物对16S rDNA基因进行PCR扩增，将该基因从多个属中扩增出来，然后进行测序。测序结果与环境中常见的细菌无色杆菌属的成员相匹配。无色杆菌已被证明对多种抗生素具有耐药性。试图净化真核细胞培养使用多种抗生素在不同浓度。使用抗生素环丙沙星和哌拉西林的组合，最终消除了污染的无色杆菌，而没有永久性地伤害真核细胞。
Cell culture model systems are utilized for their ease of use, relative inexpensiveness, and potentially limitless sample size. Reliable results cannot be obtained, however, when cultures contain contamination. This report discusses the observation and identification of mobile black specks observed in multiple cell lines. Cultures of the contamination were grown, and DNA was purified from isolated colonies. The 16S rDNA gene was PCR amplified using primers that will amplify the gene from many genera, and then sequenced. Sequencing results matched the members of the genus Achromobacter, bacteria common in the environment. Achromobacter species have been shown to be resistant to multiple antibiotics. Attempts to decontaminate the eukaryotic cell culture used multiple antibiotics at different concentrations. The contaminating Achromobacter was eventually eliminated, without permanently harming the eukaryotic cells, using a combination of the antibiotics ciprofloxacin and piperacillin.
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