Multi-omics analysis reveals gut microbiota-induced intramuscular fat deposition via regulating expression of lipogenesis-associated genes.
Multi-omics analysis reveals gut microbiota-induced intramuscular fat deposition via regulating expression of lipogenesis-associated genes.
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多组学分析揭示肠道微生物群通过调节脂肪生成相关基因的表达诱导肌内脂肪沉积
DOI：
10.1016/j.aninu.2021.10.010
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发表时间：
2022-06
期刊：
影响因子：
6.3
通讯作者：
Yan, Xianghua
中科院分区：
文献类型：
--
作者：
Xie, Chunlin;Teng, Junyong;Wang, Xinkai;Xu, Baoyang;Niu, Yaorong;Ma, Libao;Yan, Xianghua
肠道微生物组对脂质的消化、吸收和代谢有很大影响。然而，能够改变猪的脂肪沉积和肉质的微生物组成仍不清楚。在这里，我们使用莱芜猪（一种肌内脂肪较高的中国本土品种）与商业杂交杜洛克（长白×约克夏）（DLY）猪进行比较，研究微生物群对肉质，特别是肌内脂肪含量的影响。总共 32 头 DLY 仔猪被随机分配到 4 组，并移植来自健康 LW 猪的粪便微生物群。结果表明，与对照组相比，高剂量粪便微生物移植（HFMT）选择性地增强了背最长肌的脂肪沉积（P < 0.05），但降低了背膘厚度（P < 0.05）。 HFMT 显着改变肉色并提高饲料转化率 (P < 0.05)。此外，多组学分析表明，Bacteroides Uniformis、Sphaerochaeta globosa、Hydrogenoanaerobacters saccharovorans 和 Pyramidobacter piscolens 是调节脂质沉积的核心菌种。总共 140 只雄性 SPF C57BL/6j 小鼠被随机分为 7 组，并单独或联合施用这 4 种微生物，以验证微生物群与脂质沉积之间的关系。与对照小鼠相比，将细菌群落接种到小鼠体内会增加肌内脂肪含量（P < 0.05）。与对照小鼠相比，混合细菌小鼠的骨骼肌中观察到脂肪生成相关基因的表达增加，包括分化簇 36 (Cd36)、二酰基甘油 O-酰基转移酶 2 (Dgat2) 和脂肪酸合酶 (FASN)。总之，我们的结果表明肠道微生物群可能在调节猪和小鼠肌肉中的脂质沉积方面发挥重要作用。
The gut microbiome has great effects on the digestion, absorption, and metabolism of lipids. However, the microbiota composition that can alter the fat deposition and the meat quality of pigs remains unclear. Here, we used Laiwu (LW) pigs (a native Chinese breed with higher intramuscular fat) compared with commercial crossbreed Duroc × (Landrace × Yorkshire) (DLY) pigs to investigate the effects of microbiota on meat quality, especially in intramuscular fat content. A total of 32 DLY piglets were randomly allotted to 4 groups and transplanted with fecal microbiota from healthy LW pigs. The results indicated that the high dose of fecal microbiota transplantation (HFMT) selectively enhanced fat deposition in longissimus dorsi (P < 0.05) but decreased backfat thickness (P < 0.05) compared with control group. HFMT significantly altered meat color and increased feed conversation ratio (P < 0.05). Furthermore, the multi-omics analysis revealed that Bacteroides uniformis, Sphaerochaeta globosa, Hydrogenoanaerobacterium saccharovorans, and Pyramidobacter piscolens are the core species which can regulate lipid deposition. A total of 140 male SPF C57BL/6j mice were randomly allotted into 7 groups and administrated with these 4 microbes alone or consortium to validate the relationships between microbiota and lipid deposition. Inoculating the bacterial consortium into mice increased intramuscular fat content (P < 0.05) compared with control mice. Increased expressions of lipogenesis-associated genes including cluster of differentiation 36 (Cd36), diacylglycerol O-acyltransferase 2 (Dgat2), and fatty acid synthase (FASN) were observed in skeletal muscle in the mice with mixed bacteria compared with control mice. Together, our results suggest that the gut microbiota may play an important role in regulating the lipid deposition in the muscle of pigs and mice.
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昼夜主义代谢的地图集揭示了时钟之间全系统的协调和通信。
影响因子：
64.5
作者：
Dyar KA;Lutter D;Artati A;Ceglia NJ;Liu Y;Armenta D;Jastroch M;Schneider S;de Mateo S;Cervantes M;Abbondante S;Tognini P;Orozco-Solis R;Kinouchi K;Wang C;Swerdloff R;Nadeef S;Masri S;Magistretti P;Orlando V;Borrelli E;Uhlenhaut NH;Baldi P;Adamski J;Tschöp MH;Eckel-Mahan K;Sassone-Corsi P
通讯作者：
Sassone-Corsi P
影响因子：
5.2
作者：
Hu J;Nie Y;Chen J;Zhang Y;Wang Z;Fan Q;Yan X
通讯作者：
Yan X
影响因子：
--
作者：
Choi JS;Lee HJ;Jin SK;Choi YI;Lee JJ
通讯作者：
Lee JJ
影响因子：
64.8
作者：
Claesson, Marcus J.;Jeffery, Ian B.;O'Toole, Paul W.
通讯作者：
O'Toole, Paul W.
影响因子：
5
作者：
Harata, Gaku;Kumar, Himanshu;Salminen, Seppo
通讯作者：
Salminen, Seppo