A systems biology approach investigating the effect of probiotics on the vaginal microbiome and host responses in a double blind, placebo-controlled clinical trial of post-menopausal women.
A systems biology approach investigating the effect of probiotics on the vaginal microbiome and host responses in a double blind, placebo-controlled clinical trial of post-menopausal women.
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DOI：
10.1371/journal.pone.0104511
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Reid G
中科院分区：
文献类型：
--
作者：
Bisanz JE;Seney S;McMillan A;Vongsa R;Koenig D;Wong L;Dvoracek B;Gloor GB;Sumarah M;Ford B;Herman D;Burton JP;Reid G
在大多数绝经前和绝经后的妇女中，乳杆菌占主导地位的微生物区系是阴道健康的指标。这项双盲、安慰剂对照的交叉研究的目的是评估14名Nugent评分中等的绝经后妇女经阴道注射益生菌鼠李糖GR-1和路氏乳杆菌RC-14(各2.5×109CFU)对微生物区系和宿主反应的影响。与安慰剂相比，益生菌治疗并没有导致Nugent评分的改善。使用16S rRNA测序和代谢组学图谱的分析表明，与安慰剂相比，服用益生菌后乳杆菌的相对丰度增加，这与乳酸水平的增加关系不大。Atopobium的含量也出现了下降。基因芯片对宿主反应的分析表明，益生菌具有免疫调节作用，包括对TLR2等模式识别受体的影响，同时也影响上皮屏障功能。这是首次使用截骨方法研究绝经后妇女的阴道益生菌给药。这表明，在某些情况下，需要多方面的方法来检测由宿主注入益生菌菌株而引起的微妙的分子变化。ClinicalTrials.gov NCT02139839
A lactobacilli dominated microbiota in most pre and post-menopausal women is an indicator of vaginal health. The objective of this double blinded, placebo-controlled crossover study was to evaluate in 14 post-menopausal women with an intermediate Nugent score, the effect of 3 days of vaginal administration of probiotic L. rhamnosus GR-1 and L. reuteri RC-14 (2.5×109 CFU each) on the microbiota and host response. The probiotic treatment did not result in an improved Nugent score when compared to when placebo. Analysis using 16S rRNA sequencing and metabolomics profiling revealed that the relative abundance of Lactobacillus was increased following probiotic administration as compared to placebo, which was weakly associated with an increase in lactate levels. A decrease in Atopobium was also observed. Analysis of host responses by microarray showed the probiotics had an immune-modulatory response including effects on pattern recognition receptors such as TLR2 while also affecting epithelial barrier function. This is the first study to use an interactomic approach for the study of vaginal probiotic administration in post-menopausal women. It shows that in some cases multifaceted approaches are required to detect the subtle molecular changes induced by the host to instillation of probiotic strains. ClinicalTrials.gov NCT02139839
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影响因子：
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影响因子：
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10.1016/j.ijgo.2010.07.008
发表时间：
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