Adult ovine chondrocytes in expansion culture adopt progenitor cell properties that are favorable for cartilage tissue engineering.
Adult ovine chondrocytes in expansion culture adopt progenitor cell properties that are favorable for cartilage tissue engineering.
复制标题
DOI：
10.1002/jor.24671
复制
发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
Goodrich LR
中科院分区：
文献类型：
--
作者：
Kisiday JD;Liebig BE;Goodrich LR
人软骨细胞在扩增培养中可以成为祖细胞样的广泛增殖能力和分泌新软骨的软骨细胞培养。绵羊被用作软骨组织工程的大型动物模型，尽管对于测试祖细胞样软骨细胞来说，绵羊软骨细胞在采用祖细胞特性的能力方面与人类相似是重要的。在这里，我们调查绵羊软骨细胞是否可以采用祖细胞的特性所示的快速增殖，在一个集落形成的方式，和高水平的新软骨分泌的软骨培养。在已知促进间充质基质细胞扩增的条件下，绵羊软骨细胞在10天内通过约12个群体增殖。延时成像显示快速增殖的集落形成模式。扩大绵羊软骨细胞接种到琼脂糖和培养在软骨形成培养基中积累新软骨超过2周，在更大程度上比原代软骨细胞。这些数据证实，绵羊软骨细胞在获得祖细胞特性的能力方面与人类软骨细胞相似，这对于软骨组织工程非常重要。鉴于广泛的兴趣，使用祖细胞愈合结缔组织，接下来，我们比较了绵羊软骨细胞，半月板细胞和肌腱细胞的增殖和三系分化。半月板细胞和腱细胞在10天内经历了13次以上的群体倍增。在软骨形成培养中，软骨基质积累和基因表达在细胞类型之间基本相似。所有类型的细胞抵抗成骨，而扩大肌腱细胞和肌腱细胞能够脂肪形成。虽然绵羊结缔组织细胞表现出有限的谱系可塑性，但这些数据支持通过扩增促进某些祖细胞特性的潜力。
Human chondrocytes in expansion culture can become progenitor-like in their ability to proliferate extensively and secrete neocartilage in chondrogenic culture. Sheep are used as a large animal model for cartilage tissue engineering, although for testing progenitor-like chondrocytes it is important that ovine chondrocytes resemble human in the ability to adopt progenitor properties. Here, we investigate whether ovine chondrocytes can adopt progenitor properties as indicated by rapid proliferation in a colony-forming fashion, and high levels of neocartilage secretion in chondrogenic culture. In conditions known to promote expansion of mesenchymal stromal cells, ovine chondrocytes proliferated through approximately 12 population doublings in 10 days. Time-lapse imaging indicated rapid proliferation in a colony-forming pattern. Expanded ovine chondrocytes that were seeded into agarose and cultured in chondrogenic medium accumulated neocartilage over 2 weeks, to a greater extent than primary chondrocytes. These data confirm that ovine chondrocytes resemble human chondrocytes in their ability to acquire progenitor properties that are important for cartilage tissue engineering. Given the broad interest in using progenitor cells to heal connective tissues, next we compared proliferation and trilineage differentiation of ovine chondrocytes, meniscus cells, and tenocytes. Meniscus cells and tenocytes experienced more than 13 population doublings in 10 days. In chondrogenic culture, cartilage matrix accumulation, and gene expression were largely similar among the cell types. All cell types resisted osteogenesis, while expanded tenocytes and meniscal cells were capable of adipogenesis. While ovine connective tissue cells demonstrated limited lineage plasticity, these data support the potential to promote certain progenitor properties with expansion.
登录
查看更多内容
来自定型软骨细胞的人软骨来源的祖细胞，用于有效的软骨修复和再生
影响因子：
6
作者：
Jiang Y;Cai Y;Zhang W;Yin Z;Hu C;Tong T;Lu P;Zhang S;Neculai D;Tuan RS;Ouyang HW
通讯作者：
Ouyang HW
影响因子：
82.9
作者：
Bi, Yanming;Ehirchiou, Driss;Young, Marian F.
通讯作者：
Young, Marian F.
影响因子：
4.8
作者：
Bianchi, Vanessa J.;Lee, Adrienne;Kandel, Rita
通讯作者：
Kandel, Rita
影响因子：
2.8
作者：
Kisiday, John D.;Kopesky, Paul W.;Frisbie, David D.
通讯作者：
Frisbie, David D.
影响因子：
3.3
作者：
Kisiday, John D.;Hale, Benjamin W.;Frisbie, David D.
通讯作者：
Frisbie, David D.