Metabarcoding Analysis of Fungal Diversity in the Phyllosphere and Carposphere of Olive (Olea europaea).
Metabarcoding Analysis of Fungal Diversity in the Phyllosphere and Carposphere of Olive (Olea europaea).
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DOI：
10.1371/journal.pone.0131069
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Schena L
中科院分区：
文献类型：
--
作者：
Abdelfattah A;Li Destri Nicosia MG;Cacciola SO;Droby S;Schena L
使用实施的元条形码方法在不同物候阶段（五月、六月、十月和十二月）调查了与橄榄（Olea europaea）叶、花和果实相关的真菌多样性。它包括真菌 ITS2 区域的 454 焦磷酸测序以及随后相关属的系统发育分析以及经过验证的参考序列。大多数序列都在物种水平上得到了鉴定，或者与有限数量的相关分类群相关，从而支持了对其生物学作用的推测。分析揭示了一个包含 195 个不同 OTU 的丰富真菌群落。子囊菌门是主要门，占检测到的序列总数的 93.6%，其次是未鉴定的真菌 (3.6%) 和担子菌门 (2.8%)。与花和果实相比，叶子具有更高水平的多样性。在植物病原体中，以炭疽菌属（C. godetiae syn. C. clavatum、C. acutatum s.s 和 C. karstii）三个物种为代表的炭疽病菌在成熟果实上含量最多，但在其他器官中也检测到。枝孢假尾孢在所有叶子样品中检测到的频率较高，而在成熟果实中检测到的频率较低。 Spilocea oleagina 和其他假定的病原体（包括镰刀菌属、新梭孢菌属和链格孢属）的相对频率要低得多。在非病原体类群中，出芽短梗霉、枝孢枝孢属和 Devriesia spp 的物种复合体。是最具代表性的。这项研究强调了复杂的真菌群落的存在，包括植物病原微生物和潜在的拮抗微生物，它们可能对橄榄生产产生重大影响。
The fungal diversity associated with leaves, flowers and fruits of olive (Olea europaea) was investigated in different phenological stages (May, June, October and December) using an implemented metabarcoding approach. It consisted of the 454 pyrosequencing of the fungal ITS2 region and the subsequent phylogenetic analysis of relevant genera along with validated reference sequences. Most sequences were identified up to the species level or were associated with a restricted number of related taxa enabling supported speculations regarding their biological role. Analyses revealed a rich fungal community with 195 different OTUs. Ascomycota was the dominating phyla representing 93.6% of the total number of detected sequences followed by unidentified fungi (3.6%) and Basidiomycota (2.8%). A higher level of diversity was revealed for leaves compared to flowers and fruits. Among plant pathogens the genus Colletotrichum represented by three species (C. godetiae syn. C. clavatum, C. acutatum s.s and C. karstii) was the most abundant on ripe fruits but it was also detected in other organs. Pseudocercospora cladosporioides was detected with a high frequency in all leaf samples and to a less extent in ripe fruits. A much lower relative frequency was revealed for Spilocaea oleagina and for other putative pathogens including Fusarium spp., Neofusicoccum spp., and Alternaria spp. Among non-pathogen taxa, Aureobasidium pullulans, the species complex of Cladosporium cladosporioides and Devriesia spp. were the most represented. This study highlights the existence of a complex fungal consortium including both phytopathogenic and potentially antagonistic microorganisms that can have a significant impact on olive productions.
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影响因子：
16.5
作者：
Crous PW;Schoch CL;Hyde KD;Wood AR;Gueidan C;de Hoog GS;Groenewald JZ
通讯作者：
Groenewald JZ
伪cospora中的系统发育谱系。
影响因子：
16.5
作者：
Crous PW;Braun U;Hunter GC;Wingfield MJ;Verkley GJ;Shin HD;Nakashima C;Groenewald JZ
通讯作者：
Groenewald JZ
影响因子：
16.5
作者：
Cannon PF;Damm U;Johnston PR;Weir BS
通讯作者：
Weir BS
影响因子：
2.8
作者：
ANDREWS, JH;HARRIS, RF;NORDHEIM, EV
通讯作者：
NORDHEIM, EV
影响因子：
16.5
作者：
Damm U;Cannon PF;Woudenberg JH;Crous PW
通讯作者：
Crous PW