Effects of orange juice formulation on prebiotic functionality using an in vitro colonic model system.
Effects of orange juice formulation on prebiotic functionality using an in vitro colonic model system.
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DOI：
10.1371/journal.pone.0121955
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Gibson GR
中科院分区：
文献类型：
--
作者：
Costabile A;Walton GE;Tzortzis G;Vulevic J;Charalampopoulos D;Gibson GR
采用三级连续发酵结肠模型系统，在体外监测不同橙子汁配方对益生元活性的影响。三种不同的果汁，有和没有Bimuno，一种含有低聚半乳糖（B-GOS）的GOS混合物进行了评估，在他们的能力，以诱导生物合成的微生物群。配方开发基于将2.75g B-GOS掺入250 ml份的果汁（65°Brix的浓缩果汁）中。在生产B-GOS果汁的同时，开发了对照果汁-不含任何额外的Bimuno的橙子果汁和阳性对照果汁，其含有除B-GOS之外的相同相对比例的Bimuno的所有组分（葡萄糖、半乳糖和乳糖）。离子交换色谱分析用于测试生产过程后bimuno组件的维护。数据显示，灭菌对B-GOS和单糖的浓度没有显著影响。在类似于胃和小肠环境的条件下消化三种果汁制剂。在整个结肠模型研究中，使用基于16 S rRNA的荧光原位杂交（FISH）评价粪便微生物群的主要细菌群。研究了果汁补充对微生物代谢的潜在影响，使用气相色谱法测量短链脂肪酸（SCFAs）。此外，B-GOS果汁显示出对微生物群组成和代谢活性的积极调节。特别是，粪便双歧杆菌和乳酸杆菌的数量显着较高时，B-GOS果汁发酵相比，控制。此外，B-GOS果汁发酵导致罗斯拜瑞氏菌亚群的增加，并伴随着丁酸盐产量的增加，这对宿主具有潜在的益处。总之，本研究表明，橙子汁中的B-GOS对粪便微生物群具有有益作用。
A three-stage continuous fermentative colonic model system was used to monitor in vitro the effect of different orange juice formulations on prebiotic activity. Three different juices with and without Bimuno, a GOS mixture containing galactooligosaccharides (B-GOS) were assessed in terms of their ability to induce a bifidogenic microbiota. The recipe development was based on incorporating 2.75g B-GOS into a 250 ml serving of juice (65°Brix of concentrate juice). Alongside the production of B-GOS juice, a control juice – orange juice without any additional Bimuno and a positive control juice, containing all the components of Bimuno (glucose, galactose and lactose) in the same relative proportions with the exception of B-GOS were developed. Ion Exchange Chromotography analysis was used to test the maintenance of bimuno components after the production process. Data showed that sterilisation had no significant effect on concentration of B-GOS and simple sugars. The three juice formulations were digested under conditions resembling the gastric and small intestinal environments. Main bacterial groups of the faecal microbiota were evaluated throughout the colonic model study using 16S rRNA-based fluorescence in situ hybridization (FISH). Potential effects of supplementation of the juices on microbial metabolism were studied measuring short chain fatty acids (SCFAs) using gas chromatography. Furthermore, B-GOS juices showed positive modulations of the microbiota composition and metabolic activity. In particular, numbers of faecal bifidobacteria and lactobacilli were significantly higher when B-GOS juice was fermented compared to controls. Furthermore, fermentation of B-GOS juice resulted in an increase in Roseburia subcluster and concomitantly increased butyrate production, which is of potential benefit to the host. In conclusion, this study has shown B-GOS within orange juice can have a beneficial effect on the fecal microbiota.
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DOI：
10.1111/j.1365-2672.1994.tb03443.x
发表时间：
1994-10-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
GIBSON, GR;WANG, X
通讯作者：
WANG, X
影响因子：
5.2
作者：
Maccaferri, Simone;Klinder, Annett;Costabile, Adele
通讯作者：
Costabile, Adele
DOI：
10.1097/mpg.0b013e31817b6dd2
发表时间：
2009-01-01
影响因子：
2.9
作者：
Fanaro, Silvia;Marten, Berit;Bertino, Enrico
通讯作者：
Bertino, Enrico
影响因子：
4.2
作者：
Lupton, JR
通讯作者：
Lupton, JR
影响因子：
2.8
作者：
Manz, W;Amann, R;Schleifer, KH
通讯作者：
Schleifer, KH