Turnover rate of tear-film lipid layer determined by fluorophotometry.
Turnover rate of tear-film lipid layer determined by fluorophotometry.
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DOI：
10.1136/bjo.2008.156828
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发表时间：
2009-11
期刊：
影响因子：
--
通讯作者：
Tsubota K
中科院分区：
文献类型：
--
作者：
Mochizuki H;Yamada M;Hatou S;Tsubota K
进行本研究以独立地评估泪膜的水层和脂质层的周转率。两种荧光染料，荧光素钠和5-十二烷酰氨基荧光素（5-dodecanoylaminofluorescein，5-dodecanoylaminofluorescein），这是一种游离脂肪酸共轭的荧光素，应用于12名健康志愿者的右眼。荧光素钠每分钟在中央角膜处测量角膜前泪膜的荧光强度，持续10分钟，荧光素钠每5分钟在中央角膜处测量角膜前泪膜的荧光强度，持续50分钟。通过在半对数图中绘制荧光强度对时间的曲线来计算周转率，并表示为%/min。荧光素钠和荧光素钠的周转率分别为10.3（SD 3.7）%/min和0.93（0.36）%/min。荧光素钠的转换率显著低于荧光素钠（p<0.05，Mann-Whitney检验）。荧光素钠与荧光素钠的转换率呈正相关（r = 0.93，p<0.05）。 我们的研究结果表明，泪液中脂质的周转比泪液的水相流动慢得多，并且这种脂质周转与健康成人的泪液的水相流动有关。
This study was performed to independently assess the turnover rates of aqueous and lipid layers of the tear film. Two fluorescent dyes, fluorescein sodium and 5-dodecanoylaminofluorescein (DAF), which is a free-fatty-acid conjugate of fluorescein, were applied to the right eye of 12 healthy volunteers. Fluorescent intensity of the precorneal tear film was measured at the central cornea every minute for 10 min for fluorescein sodium, and every 5 min for 50 min for DAF. The turnover rate was calculated by plotting fluorescent intensity against time in a semilog plot and expressed as %/min. Turnover rates of fluorescein sodium and DAF were 10.3 (SD 3.7)%/min and 0.93 (0.36)%/min, respectively. The turnover rate of DAF was significantly lower than that of fluorescein sodium (p<0.05, Mann–Whitney test). The turnover rate of DAF positively correlated with that of fluorescein sodium (r = 0.93, p<0.05). Our results indicate that the turnover of lipids in tears is much slower than the aqueous flow of tears, and that this lipid turnover is associated with the aqueous flow of tears in healthy adults.
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影响因子：
1.4
作者：
Craig, JP;Tomlinson, A
通讯作者：
Tomlinson, A
影响因子：
2.8
作者：
KORB, DR;BARON, DF;GREINER, JV
通讯作者：
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影响因子：
4.1
作者：
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影响因子：
3.4
作者：
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影响因子：
3.4
作者：
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通讯作者：
REFOJO, MF