Preparation and Characterization of Covalently Binding of Rat Anti-human IgG Monolayer on Thiol-Modified Gold Surface.
Preparation and Characterization of Covalently Binding of Rat Anti-human IgG Monolayer on Thiol-Modified Gold Surface.
复制标题
硫醇修饰金表面共价结合的大鼠抗人 IgG 单分子层的制备和表征
DOI：
10.1007/s11671-009-9412-9
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发表时间：
2009-09-16
影响因子：
--
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Lv Z;Wang J;Deng L;Chen G
采用自组装单层膜（SAM）技术在金基底上制备了16-巯基十六烷酸（MHA）膜和大鼠抗人IgG蛋白单层膜。分别采用接触角测量、原子力显微镜（AFM）、掠入射X射线衍射（GIXRD）和X射线光电子能谱（XPS）对裸金基底、MHA膜和蛋白质单层膜的表面性质进行了表征。MHA膜和蛋白质单层的接触角分别为18°和12°，均为亲水性。AFM图像显示不同的表面之间的不同的地形纳米结构，和MHA膜和蛋白质单层的厚度估计分别为1.51和5.53 nm。MHA膜和蛋白质单分子层的GIXRD 2θ度范围为0° ~ 15°，明显小于裸金表面的GIXRD 2 θ度，但MHA膜和蛋白质单分子层的衍射峰的轮廓和分布有很大不同。此外，从这些不同的表面测量的结合能的光谱可以很好地拟合与Au 4f，S2 p或N1s，分别。总之，这些结果表明，MHA膜和蛋白质单层成功地形成了均匀的表面，从而证明SAM方法是一种可靠的技术，用于制造蛋白质单层。
The 16-mercaptohexadecanoic acid (MHA) film and rat anti-human IgG protein monolayer were fabricated on gold substrates using self-assembled monolayer (SAM) method. The surface properties of the bare gold substrate, the MHA film and the protein monolayer were characterized by contact angle measurements, atomic force microscopy (AFM), grazing incidence X-ray diffraction (GIXRD) method and X-ray photoelectron spectroscopy, respectively. The contact angles of the MHA film and the protein monolayer were 18° and 12°, respectively, all being hydrophilic. AFM images show dissimilar topographic nanostructures between different surfaces, and the thickness of the MHA film and the protein monolayer was estimated to be 1.51 and 5.53 nm, respectively. The GIXRD 2θ degrees of the MHA film and the protein monolayer ranged from 0° to 15°, significantly smaller than that of the bare gold surface, but the MHA film and the protein monolayer displayed very different profiles and distributions of their diffraction peaks. Moreover, the spectra of binding energy measured from these different surfaces could be well fitted with either Au4f, S2p or N1s, respectively. Taken together, these results indicate that MHA film and protein monolayer were successfully formed with homogeneous surfaces, and thus demonstrate that the SAM method is a reliable technique for fabricating protein monolayer.
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影响因子：
3.9
作者：
Ishida, T;Tsuneda, S;Knoll, W
通讯作者：
Knoll, W
影响因子：
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作者：
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作者：
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影响因子：
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作者：
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3.9
作者：
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通讯作者：
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