Multidendritic sensory neurons in the adult Drosophila abdomen: origins, dendritic morphology, and segment- and age-dependent programmed cell death.
Multidendritic sensory neurons in the adult Drosophila abdomen: origins, dendritic morphology, and segment- and age-dependent programmed cell death.
复制标题
DOI：
10.1186/1749-8104-4-37
复制
发表时间：
2009-10-02
影响因子：
3.6
通讯作者：
Uemura T
中科院分区：
文献类型：
--
作者：
Shimono K;Fujimoto A;Tsuyama T;Yamamoto-Kochi M;Sato M;Hattori Y;Sugimura K;Usui T;Kimura K;Uemura T
为了建立支持各种动物行为的功能性神经回路，必须重组早期发育中形成的初始回路。实现这一点的一种方法是对电路硬接线进行局部重构。为了从遗传学上研究这种重塑的潜在机制，一个模型系统采用了一组主要的果蝇多树突感觉神经元-树突状树枝化（da）神经元-这表现出显着的树突修剪和随后的增长在变态。15 DA神经元被确定在每个幼虫腹部hemisegment，并被分为四类-类I至IV-为了增加其感受野的大小和/或乔木的复杂性在成熟的幼虫阶段。我们对成年多巴胺能神经元的解剖学和发育基础的了解仍然是零碎的。我们在成年果蝇腹部发现了多树突状神经元，观察了单个神经元的树突状结构，并将这些细胞的起源追溯到幼虫阶段。成虫腹半节3或4（A3/4）有6个da神经元，其中5个为持久性幼虫da神经元。我们定量分析了6个成人神经元中的3个的树突状乔木，并研究了导致幼虫类选择性树突状形态的关键转录因子在pharate成人中的表达。成人A3/4的"基线设计"以节段依赖性和年龄依赖性方式进一步修改。我们的一个显着的发现是，幼虫I类神经元，ddaE，完成树突状重塑A2至A4，然后在羽化后1周内进行半胱天冬酶依赖性细胞死亡，而同源神经元在A5和更后段退化蛹阶段。另一个发现是，IV类神经元v'ada的树突状乔木在羽化后的生长过程中立即重塑。它表现出突出的径向晶格转换在1日龄的成年人，并由此产生的晶格状乔木持续整个成年生活。我们的研究提供了基础，我们可以调查的遗传程序控制树突重塑和程序性细胞死亡的成年神经元，和树突乔木的终身维护。
For the establishment of functional neural circuits that support a wide range of animal behaviors, initial circuits formed in early development have to be reorganized. One way to achieve this is local remodeling of the circuitry hardwiring. To genetically investigate the underlying mechanisms of this remodeling, one model system employs a major group of Drosophila multidendritic sensory neurons - the dendritic arborization (da) neurons - which exhibit dramatic dendritic pruning and subsequent growth during metamorphosis. The 15 da neurons are identified in each larval abdominal hemisegment and are classified into four categories - classes I to IV - in order of increasing size of their receptive fields and/or arbor complexity at the mature larval stage. Our knowledge regarding the anatomy and developmental basis of adult da neurons is still fragmentary. We identified multidendritic neurons in the adult Drosophila abdomen, visualized the dendritic arbors of the individual neurons, and traced the origins of those cells back to the larval stage. There were six da neurons in abdominal hemisegment 3 or 4 (A3/4) of the pharate adult and the adult just after eclosion, five of which were persistent larval da neurons. We quantitatively analyzed dendritic arbors of three of the six adult neurons and examined expression in the pharate adult of key transcription factors that result in the larval class-selective dendritic morphologies. The 'baseline design' of A3/4 in the adult was further modified in a segment-dependent and age-dependent manner. One of our notable findings is that a larval class I neuron, ddaE, completed dendritic remodeling in A2 to A4 and then underwent caspase-dependent cell death within 1 week after eclosion, while homologous neurons in A5 and in more posterior segments degenerated at pupal stages. Another finding is that the dendritic arbor of a class IV neuron, v'ada, was immediately reshaped during post-eclosion growth. It exhibited prominent radial-to-lattice transformation in 1-day-old adults, and the resultant lattice-shaped arbor persisted throughout adult life. Our study provides the basis on which we can investigate the genetic programs controlling dendritic remodeling and programmed cell death of adult neurons, and the life-long maintenance of dendritic arbors.
登录
查看更多内容
影响因子：
10.5
作者：
Kim, Michael D.;Jan, Lily Yeh;Jan, Yuh Nung
通讯作者：
Jan, Yuh Nung
影响因子：
2.1
作者：
Hattori, Yukako;Sugimura, Kaoru;Uemura, Tadashi
通讯作者：
Uemura, Tadashi
影响因子：
2.5
作者：
Dulcis, D;Levine, RB
通讯作者：
Levine, RB
影响因子：
9.2
作者：
Ainsley, JA;Pettus, JM;Johnson, WA
通讯作者：
Johnson, WA
影响因子：
64.8
作者：
Emoto, Kazuo;Parrish, Jay Z.;Jan, Yuh-Nung
通讯作者：
Jan, Yuh-Nung