The effect of pulsed electromagnetic field exposure on osteoinduction of human mesenchymal stem cells cultured on nano-TiO2 surfaces.
The effect of pulsed electromagnetic field exposure on osteoinduction of human mesenchymal stem cells cultured on nano-TiO2 surfaces.
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DOI：
10.1371/journal.pone.0199046
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Visai L
中科院分区：
文献类型：
--
作者：
Bloise N;Petecchia L;Ceccarelli G;Fassina L;Usai C;Bertoglio F;Balli M;Vassalli M;Cusella De Angelis MG;Gavazzo P;Imbriani M;Visai L
人骨髓间充质干细胞（hBM-MSCs）被认为在骨修复和再生方面具有很大的前景。大量的努力都是为了发现促进干细胞成骨分化的最佳策略。在之前的研究中，hBM-MSCs暴露于物理刺激，如脉冲电磁场（pemf）或直接在纳米结构的钛表面（TiO2）上种子，被证明可以促进成骨条件下向成骨细胞的分化。在本研究中，研究了每日暴露于pemf对hBM-MSCs在纳米结构TiO2（粒径小于100 nm）上的成骨分化的影响。将tio2种子细胞暴露于PEMF（磁场强度：2 mT；感应电场强度：5 mV；频率：75 Hz）中，并检查细胞生理改变和成骨分化。结果表明，PEMF暴露通过干扰选择性钙相关成骨途径影响tio2种子细胞的成骨，并大大增强hBM-MSCs的成骨特征，如早期/晚期成骨基因的表达、蛋白质的产生（如ALP、COL-I、骨钙素和骨桥蛋白）和ALP活性。最后，经pemf处理的细胞比未经处理的细胞在条件培养基中分泌更多的BMP-2、DCN和col - 1。这些发现再次证实了PEMF的成骨诱导潜力，表明其与TiO2纳米结构表面的结合可能是骨组织工程应用的一个很好的选择。
Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are considered a great promise in the repair and regeneration of bone. Considerable efforts have been oriented towards uncovering the best strategy to promote stem cells osteogenic differentiation. In previous studies, hBM-MSCs exposed to physical stimuli such as pulsed electromagnetic fields (PEMFs) or directly seeded on nanostructured titanium surfaces (TiO2) were shown to improve their differentiation to osteoblasts in osteogenic condition. In the present study, the effect of a daily PEMF-exposure on osteogenic differentiation of hBM-MSCs seeded onto nanostructured TiO2 (with clusters under 100 nm of dimension) was investigated. TiO2-seeded cells were exposed to PEMF (magnetic field intensity: 2 mT; intensity of induced electric field: 5 mV; frequency: 75 Hz) and examined in terms of cell physiology modifications and osteogenic differentiation. Results showed that PEMF exposure affected TiO2-seeded cells osteogenesis by interfering with selective calcium-related osteogenic pathways, and greatly enhanced hBM-MSCs osteogenic features such as the expression of early/late osteogenic genes and protein production (e.g., ALP, COL-I, osteocalcin and osteopontin) and ALP activity. Finally, PEMF-treated cells resulted to secrete into conditioned media higher amounts of BMP-2, DCN and COL-I than untreated cell cultures. These findings confirm once more the osteoinductive potential of PEMF, suggesting that its combination with TiO2 nanostructured surface might be a great option in bone tissue engineering applications.
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影响因子：
14
作者：
Awad, HA;Wickham, MQ;Guilak, F
通讯作者：
Guilak, F
影响因子：
5.6
作者：
Bernardo, M. E.;Avanzini, M. A.;Locatelli, F.
通讯作者：
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影响因子：
14
作者：
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通讯作者：
Papaccio, Gianpaolo
影响因子：
7.5
作者：
Maziarz A;Kocan B;Bester M;Budzik S;Cholewa M;Ochiya T;Banas A
通讯作者：
Banas A
影响因子：
4.1
作者：
Addison WN;Nelea V;Chicatun F;Chien YC;Tran-Khanh N;Buschmann MD;Nazhat SN;Kaartinen MT;Vali H;Tecklenburg MM;Franceschi RT;McKee MD
通讯作者：
McKee MD