Insight into the structural organization of the omega leader of TMV RNA: the role of various regions of the sequence in the formation of a compact structure of the omega RNA.
Insight into the structural organization of the omega leader of TMV RNA: the role of various regions of the sequence in the formation of a compact structure of the omega RNA.
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DOI：
10.1016/j.bbrc.2010.11.102
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发表时间：
2011-01-07
影响因子：
3.1
通讯作者：
Spirin AS
中科院分区：
文献类型：
--
作者：
Agalarov SC;Sogorin EA;Shirokikh NE;Spirin AS
烟草花叶病毒RNA的5'-未翻译序列-即所谓的ω先导-是一个众所周知的翻译增强子。RNA的结构有不同寻常的特点。尽管缺乏广泛的沃森-克里克型二级结构，但omega RNA具有稳定的致密构象。omega序列的中心部分包含许多CAA重复序列，两侧是富u区。本文合成了含有修饰omega序列的多核糖核苷酸，并利用分析超离心和热熔技术对其进行了研究。结果表明，在序列的中心和3'-近端部分发生的变化导致omega RNA结构的强烈不稳定。我们得出结论，规则（CAA）n核心区域和omega RNA的3'-近端富au区域相互作用，共同有助于形成稳定的三级结构。
The 5'-untranslated sequence of tobacco mosaic virus RNA – the so called omega leader – is a well-known translational enhancer. The structure of the omega RNA has unusual features. Despite the absence of extensive secondary structure of the Watson-Crick type, the omega RNA possesses a stable compact conformation. The central part of the omega sequence contains many CAA repeats and is flanked by U-rich regions. In this work we synthesized the polyribonucleotides containing modified omega sequences, and studied them using analytical ultracentrifugation and thermal melting techniques. It was demonstrated that changes made in both the central and the 3'-proximal part of the sequence led to a strong destabilization of the omega RNA structure. We conclude that the regular (CAA)n core region and the 3'-proximal AU-rich region of the omega RNA interact with each other and contribute together to the formation of a stable tertiary structure.
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影响因子：
14.9
作者：
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通讯作者：
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影响因子：
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DOI：
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发表时间：
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影响因子：
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作者：
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通讯作者：
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