Immunohistochemical analysis of IL-6, IL-8/CXCR2 axis, Tyr p-STAT-3, and SOCS-3 in lymph nodes from patients with chronic lymphocytic leukemia: correlation between microvascular characteristics and prognostic significance.
Immunohistochemical analysis of IL-6, IL-8/CXCR2 axis, Tyr p-STAT-3, and SOCS-3 in lymph nodes from patients with chronic lymphocytic leukemia: correlation between microvascular characteristics and prognostic significance.
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来自慢性淋巴细胞性白血病患者的淋巴结中IL-6，IL-8/CXCR2轴，Tyr P-STAT-3和SOCS-3的免疫组织化学分析：微血管特征与预后意义之间的相关性。
DOI：
10.1155/2014/251479
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发表时间：
2014
影响因子：
--
通讯作者：
Korkolopoulou P
中科院分区：
文献类型：
--
作者：
Levidou G;Sachanas S;Pangalis GA;Kalpadakis C;Yiakoumis X;Moschogiannis M;Sepsa A;Lakiotaki E;Milionis V;Kyrtsonis MC;Vassilakopoulos TP;Tsirkinidis P;Kontopidou F;Kokoris S;Siakantaris M;Angelopoulou M;Papadaki H;Kavantzas N;Panayiotidis P;Patsouris E;Korkolopoulou P
许多研究探讨了血管生成在慢性淋巴细胞性白血病中的病理生理学作用，但结果往往不一致。我们的目标是直接了解CLL参与的淋巴结血管生成过程，重点是促血管生成细胞因子和微血管形态计量学。应用免疫组织化学方法检测62例CLL/SLL组织中血管内皮生长因子、Th-2细胞因子IL-6和IL-8、IL-8受体CXCR2和酪氨酸p-STAT-3/SOCS-3轴调节细胞因子的表达。通过图像分析评价微血管特征。根据临床病理特征对结果进行分析。与非PC区相比，增殖中心(PC)的血管化程度较低。IL-8和CXCR2的表达明显不常见，而IL-6、血管内皮生长因子和SOCS-3在绝大多数病例中都能检测到。后两种分子在40%的∼患者的PC中表达更为明显。66.67%的PC患者P-STAT-3表达阳性。微血管形态计量学与促血管生成细胞因子、p-STAT-3、SOCS-3或生存期无关。微血管口径和血管内皮生长因子在A期表达较高，而IL-6在C期表达较高。在多因素分析中，血管内皮生长因子和p-STAT-3对预后有显著影响，是CLL患者潜在的预后预测因素。
A number of studies have looked into the pathophysiological role of angiogenesis in CLL, but the results have often been inconsistent. We aimed to gain direct insight into the angiogenic process in lymph nodes involved by CLL, focusing on proangiogenic cytokines and microvessel morphometry. The tissue levels of VEGF, Th-2 cytokines IL-6 and IL-8, IL-8 receptor CXCR2, and tyrosine p-STAT-3/SOCS-3 axis modulating cytokine expression were evaluated immunohistochemically in 62 CLL/SLL cases. Microvascular characteristics were evaluated by image analysis. Results were analyzed with regard to clinicopathological characteristics. Proliferation centers (PCs) were less well vascularised compared to non-PC areas. IL-8 and CXCR2 expression was distinctly uncommon as opposed to IL-6, VEGF and SOCS-3, which were detected in the vast majority of cases. The latter two molecule expressions were more pronounced in the PCs in ∼40% of the cases. p-STAT-3 immunoreactivity was recorded in 66.67% of the cases with a predilection for PCs. Microvessel morphometry was unrelated to proangiogenic cytokines, p-STAT-3, SOCS-3, or survival. Microvascular caliber and VEGF expression were higher in Binet stage A, whereasIL-6 expression was higher in stage C. VEGF and p-STAT-3 exerted a favorable effect on progression, which remained significant in multivariate analysis, thereby constituting potential outcome predictors in CLL patients.
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影响因子：
5.1
作者：
HU, DE;HORI, Y;FAN, TPD
通讯作者：
FAN, TPD
影响因子：
2.7
作者：
Aguayo, A;Manshouri, T;Albitar, M
通讯作者：
Albitar, M
影响因子：
11.4
作者：
Kini, AR;Kay, NE;Peterson, LC
通讯作者：
Peterson, LC
影响因子：
20.3
作者：
Fayad, L;Keating, MJ;Kurzrock, R
通讯作者：
Kurzrock, R
影响因子：
20.3
作者：
Aguayo, A;Kantarjian, H;Albitar, M
通讯作者：
Albitar, M