DNA as a powerful tool for morphology control, spatial positioning, and dynamic assembly of nanoparticles.
DNA as a powerful tool for morphology control, spatial positioning, and dynamic assembly of nanoparticles.
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DOI：
10.1021/ar500081k
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发表时间：
2014-06-17
影响因子：
18.3
通讯作者：
Lu, Yi
中科院分区：
文献类型：
--
作者：
Tan, Li Huey;Xing, Hang;Lu, Yi
纳米材料的多种特性，例如形态（例如形状和表面结构）和距离相关特性（例如等离子体和量子限制效应），使纳米材料成为从催化到生物医学的未来应用的唯一潜在选择。为了充分发挥这些纳米材料的潜力，重要的是要证明对单个纳米颗粒形态的精细控制，以及对多个纳米颗粒之间的位置、方向和距离的精确空间控制。此外，还需要对纳米材料组装响应多种刺激进行动态控制，误差最小或无误差，并且组装具有可逆性。在本报告中，我们总结了使用 DNA 作为强大的可编程工具来实现上述目标的最新进展。首先，受到生物学中遗传密码发现的启发，我们发现了 DNA 序列组合可以控制纳米颗粒在生长过程中的不同形态，并表明这些效应是协同的或竞争的，具体取决于序列组合。引导纳米材料生长的DNA是稳定的并保留其生物识别能力。其次，通过利用硫代磷酸酯和磷酸二酯主链的不同反应性，我们将硫代磷酸酯放置在包括DNA四面体在内的不同DNA纳米结构上的选择性位置。双功能接头已用于在一端结合硫代磷酸酯，并在另一端结合纳米颗粒或蛋白质。这样做，可以实现以纳米分辨率精确控制两个或多个纳米粒子或蛋白质之间的距离。此外，通过开发简单的方法，用两种不同的DNA序列区域选择性地功能化Janus纳米颗粒的两个半球，我们已经证明了纳米材料组装的定向控制，其中具有特定杂交的DNA链充当正交接头。第三，通过使用包括DNAzyme、适体和适体酶在内的功能性DNA，实现了对金纳米颗粒、量子点、碳纳米管和氧化铁纳米颗粒组装体的动态控制，以协同响应一种或多种刺激，从而产生针对金属离子、小分子、蛋白质和完整细胞等多种靶标的比色、荧光、电化学和磁共振信号。第四，通过模仿生物学，我们使用DNAzymes作为校对单元来消除纳米粒子组装中的错误，并进一步使用DNAzyme级联反应来修改或修复组装中涉及的DNA序列。最后，通过利用生物素和脱硫生物素对链霉亲和素的不同亲和力，我们证明了蛋白质在 DNA 折纸上的可逆组装。
Several properties of nanomaterials, such as morphologies (e.g., shapes and surface structures) and distance dependent properties (e.g., plasmonic and quantum confinement effects), make nanomaterials uniquely qualified as potential choices for future applications from catalysis to biomedicine. To realize the full potential of these nanomaterials, it is important to demonstrate fine control of the morphology of individual nanoparticles, as well as precise spatial control of the position, orientation, and distances between multiple nanoparticles. In addition, dynamic control of nanomaterial assembly in response to multiple stimuli, with minimal or no error, and the reversibility of the assemblies are also required. In this Account, we summarize recent progress of using DNA as a powerful programmable tool to realize the above goals. First, inspired by the discovery of genetic codes in biology, we have discovered DNA sequence combinations to control different morphologies of nanoparticles during their growth process and have shown that these effects are synergistic or competitive, depending on the sequence combination. The DNA, which guides the growth of the nanomaterial, is stable and retains its biorecognition ability. Second, by taking advantage of different reactivities of phosphorothioate and phosphodiester backbone, we have placed phosphorothioate at selective positions on different DNA nanostructures including DNA tetrahedrons. Bifunctional linkers have been used to conjugate phosphorothioate on one end and bind nanoparticles or proteins on the other end. In doing so, precise control of distances between two or more nanoparticles or proteins with nanometer resolution can be achieved. Furthermore, by developing facile methods to functionalize two hemispheres of Janus nanoparticles with two different DNA sequences regioselectively, we have demonstrated directional control of nanomaterial assembly, where DNA strands with specific hybridization serve as orthogonal linkers. Third, by using functional DNA that includes DNAzyme, aptamer, and aptazyme, dynamic control of assemblies of gold nanoparticles, quantum dots, carbon nanotubes, and iron oxide nanoparticles in response to one or more stimuli cooperatively have been achieved, resulting in colorimetric, fluorescent, electrochemical, and magnetic resonance signals for a wide range of targets, such as metal ions, small molecules, proteins, and intact cells. Fourth, by mimicking biology, we have employed DNAzymes as proofreading units to remove errors in nanoparticle assembly and further used DNAzyme cascade reactions to modify or repair DNA sequences involved in the assembly. Finally, by taking advantage of different affinities of biotin and desthiobiotin toward streptavidin, we have demonstrated reversible assembly of proteins on DNA origami.
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影响因子：
62.1
作者：
Liu, Juewen;Cao, Zehui;Lu, Yi
通讯作者：
Lu, Yi
影响因子：
64.8
作者：
ELLINGTON, AD;SZOSTAK, JW
通讯作者：
SZOSTAK, JW
影响因子：
15
作者：
Lee, Jung Heon;Wong, Ngo Yin;Lu, Yi
通讯作者：
Lu, Yi
影响因子：
15
作者：
Lee, Jung Heon;Wang, Zidong;Liu, Juewen;Lu, Yi
通讯作者：
Lu, Yi
DOI：
10.1126/science.1227268
发表时间：
2012-11-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ke Y;Ong LL;Shih WM;Yin P
通讯作者：
Yin P