High temperatures alter physiological properties of pyramidal cells and inhibitory interneurons in hippocampus.
High temperatures alter physiological properties of pyramidal cells and inhibitory interneurons in hippocampus.
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DOI：
10.3389/fncel.2012.00027
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发表时间：
2012
影响因子：
5.3
通讯作者：
Connors BW
中科院分区：
文献类型：
--
作者：
Kim JA;Connors BW
温度对神经元有多种影响，但人们对高温对哺乳动物中枢神经元生理的影响知之甚少。体温过高会影响行为并导致热性惊厥。我们通过记录锥体神经元和抑制性定向-分子陷窝（O-LM）中间神经元（通过GIN小鼠系中的绿色荧光蛋白（GFP）表达鉴定），研究了急性高温对体外未成熟海马的影响。升温至41°C引起去极化、自发动作电位、输入电阻和膜时间常数降低，以及大多数锥体细胞和O-LM中间神经元的自发突触活动增加。高温对CA 3区锥体神经元的兴奋作用强于CA 1区。大约90%的O-LM中间神经元在CA 1和CA 3增加他们的放电率在高温下，CA 3的中间神经元比CA 1的平均放电速度更快。阻断快速突触传递并不能消除高温对神经元兴奋性的影响。我们的研究结果表明，高温增加海马的兴奋性，特别是在海马CA 3区，通过改变锥体细胞和中间神经元的固有膜特性。
Temperature has multiple effects on neurons, yet little is known about the effects of high temperature on the physiology of mammalian central neurons. Hyperthermia can influence behavior and cause febrile seizures. We studied the effects of acute hyperthermia on the immature hippocampus in vitro by recording from pyramidal neurons and inhibitory oriens-lacunosum moleculare (O-LM) interneurons (identified by green fluorescent protein (GFP) expression in the GIN mouse line). Warming to 41°C caused depolarization, spontaneous action potentials, reduced input resistance and membrane time constant, and increased spontaneous synaptic activity of most pyramidal cells and O-LM interneurons. Pyramidal neurons of area CA3 were more strongly excited by hyperthermia than those of area CA1. About 90% of O-LM interneurons in both CA1 and CA3 increased their firing rates at hyperthermic temperatures; interneurons in CA3 fired faster than those in CA1 on average. Blockade of fast synaptic transmission did not abolish the effect of hyperthermia on neuronal excitability. Our results suggest that hyperthermia increases hippocampal excitability, particularly in seizure-prone area CA3, by altering the intrinsic membrane properties of pyramidal cells and interneurons.
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影响因子：
25
作者：
Cruikshank, Scott J.;Lewis, Timothy J.;Connors, Barry W.
通讯作者：
Connors, Barry W.
影响因子：
25
作者：
Bains, JS;Longacher, JM;Staley, KJ
通讯作者：
Staley, KJ
影响因子：
6.1
作者：
Derchansky, M.;Rokni, D.;Carlen, P. L.
通讯作者：
Carlen, P. L.
影响因子：
82.9
作者：
Chen, K;Baram, TZ;Soltesz, I
通讯作者：
Soltesz, I
影响因子：
2.2
作者：
Buckmaster, PS;Otero-Corchón, V;Low, MJ
通讯作者：
Low, MJ