Antimicrobial Polymer with Enhanced Activity and Reduced Toxicity upon Grafting to Chitosan Oligosaccharide
Antimicrobial Polymer with Enhanced Activity and Reduced Toxicity upon Grafting to Chitosan Oligosaccharide
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接枝壳寡糖后活性增强、毒性降低的抗菌聚合物
DOI：
10.1007/s13369-019-04260-4
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发表时间：
2020-01
影响因子：
2.9
通讯作者：
Xia Wenshui
中科院分区：
文献类型：
--
作者：
Liu Xiaoli;Xie Wancui;Yang Xihong;Zhan Xiaobei;Xia Wenshui
本工作的目的是开发一种新型的、无毒的、生物活性的抗菌材料，该材料基于天然聚合物、壳聚糖低聚糖（COS）、天然吡咯酮化合物、曲酸（KA）和哌嗪。通过选择性部分烷基化反应，将曲酸、KA和哌嗪接枝到COS分子链上，合成了COS- o - mb聚合物。通过FT-IR、NMR、Mw、PID、抗菌活性、溶血实验、生物相容性、动物毒性和抗菌作用模式等研究，评价了cos - o - mb的理化性质和生物活性。结果表明，烷基化反应发生在COS的C-6位点，制备的COS- o - mb与游离COS和KA相比，体外抗菌活性和生物相容性显著增强，溶血活性降低。同时，抗菌作用模式研究证明，COS-O-MB可穿过阴离子微生物膜，破坏细胞膜完整性，并可能影响蛋白质变性，表明暴露于COS-O-MB可导致微生物细胞死亡。但重要的是，COS-O-MB对人体细胞没有毒性。因此，SLAC KM菌株对人红细胞的低溶血活性和对雌性小鼠的无毒特性使这种新型聚合物材料成为食品和制药工业中有前景和有效的化合物。
The aim of this work was to develop a novel, nontoxic, bioactive antimicrobial material based on a natural polymer, chitosan oligosaccharides (COS), a natural pyrone compound, kojic acid (KA), and piperazine. The COS-based polymer (COS-O-MB) has been synthesized by selective partial alkylation reaction to graft kojic acid, KA, and piperazine onto the COS molecular chain. Then, physicochemical properties and bioactivity of the COS-O-MBs were evaluated and it was characterized by FT-IR, NMR spectroscopy, Mw, PID, antimicrobial activity, hemolysis assay, biocompatibility, animal toxicity, and antimicrobial action mode studies. The results revealed that the alkylation reaction took place at the C-6 position of COS, and the as-prepared COS-O-MB significantly enhanced the antimicrobial activity and biocompatibility and reduced the hemolytic activity in vitro in comparison with that of the free COS and KA. Meanwhile, the antimicrobial action mode studies proved that COS-O-MB can pass through the anionic microbial membrane, destroy the cytomembrane integrity, and potentially affect protein denaturation, demonstrating that exposure to COS-O-MB results in the death of microbial cells. But importantly, COS-O-MB is not toxic to human cells. Hence, low hemolytic activity to human red blood cells, and nontoxic nature to female mice of SLAC KM strain, made this novel polymer material a promising and effective compound for food and pharmaceutical industries.
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影响因子：
6.2
作者：
Faig JJ;Moretti A;Joseph LB;Zhang Y;Nova MJ;Smith K;Uhrich KE
通讯作者：
Uhrich KE
影响因子：
11.2
作者：
Feilong Yan;Qifeng Dang;Chengsheng Liu;Jingquan Yan;Teng Wang;Bing Fan;Dongsu Cha;Xiaoli Li;Shengnan Liang;Zhenzhen Zhang
通讯作者：
Feilong Yan;Qifeng Dang;Chengsheng Liu;Jingquan Yan;Teng Wang;Bing Fan;Dongsu Cha;Xiaoli Li;Shengnan Liang;Zhenzhen Zhang
DOI：
10.1016/j.ijbiomac.2018.03.007
发表时间：
2018-07
影响因子：
8.2
作者：
Xiaoli Liu;Q. Jiang;W. Xia
通讯作者：
Xiaoli Liu;Q. Jiang;W. Xia
影响因子：
2.6
作者：
M. Aytemi̇r;B. Özçelik
通讯作者：
M. Aytemi̇r;B. Özçelik
DOI：
10.1016/j.bbagen.2015.12.010
发表时间：
2016-03
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
V. Petit;J. Rolland;A. Blond;C. Cazevieille;C. Djédiat;J. Péduzzi;C. Goulard;E. Bachère;J. Dupont
通讯作者：
V. Petit;J. Rolland;A. Blond;C. Cazevieille;C. Djédiat;J. Péduzzi;C. Goulard;E. Bachère;J. Dupont