Osteoblastic differentiation of human and equine adult bone marrow-derived mesenchymal stem cells when BMP-2 or BMP-7 homodimer genetic modification is compared to BMP-2/7 heterodimer genetic modification in the presence and absence of dexamethasone.
Osteoblastic differentiation of human and equine adult bone marrow-derived mesenchymal stem cells when BMP-2 or BMP-7 homodimer genetic modification is compared to BMP-2/7 heterodimer genetic modification in the presence and absence of dexamethasone.
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当BMP-2或BMP-7同型二聚体遗传修饰与BMP-2/7异二聚体遗传修饰的情况下，在存在和不存在地金塞米松的情况下，将人和马成年骨髓衍生的间充质干细胞的成骨细胞分化与BMP-2/7杂化基因修饰进行了比较。
DOI：
10.1002/jor.21126
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发表时间：
2010-10
影响因子：
2.8
通讯作者：
Hidaka, Chisa
中科院分区：
文献类型：
--
作者：
Carpenter, Ryan S.;Goodrich, Laurie R.;Frisbie, David D.;Kisiday, John D.;Carbone, Beth;McIlwraith, C. Wayne;Centeno, Christopher J.;Hidaka, Chisa
骨髓源性间充质干细胞（BMDMSCs）已被用于增强骨愈合；它们的成骨潜能可能会被编码骨形态发生蛋白（BMP）的基因进一步增强。本研究的目的是比较在地塞米松存在或不存在的情况下，BMP-2和bmp - 7或BMP-2和bmp - 7基因修饰对人和马BMDMSCs成骨分化的影响。骨髓间充质干细胞来自3名人类供体的髂骨和3名马供体的髋结节。利用编码BMP-2、-7或两者的腺病毒载体对单层细胞进行基因修饰，并在地塞米松存在或不存在的情况下进行培养。酶联免疫吸附法证实bmp的表达。为评估成骨细胞分化，每隔一天检测一次细胞形态，测定碱性磷酸酶（ALP）的表达和分泌，以及骨连接素、骨钙素和Runx2的表达水平，持续14天。人和马的骨髓间充质干细胞显示出成骨分化的能力，无论是否进行基因改造或补充地塞米松。补充地塞米松对马骨髓间充质干细胞成骨分化的影响大于人骨髓间充质干细胞。与AdBMP 7或AdBMP 2/7相比，BMDMSCs的基因修饰增加了AdBMP-2同型二聚体的ALP分泌，在人和马细胞中效果最大。BMP蛋白洗脱率在第4天至第8天达到最大浓度，此后保持相对稳定，这表明转基因BMDMSCs可用于将BMP蛋白以细胞为基础递送到骨形成部位。
Bone marrow-derived mesenchymal stem cells (BMDMSCs) have been targeted for use in enhancement of bone healing; and their osteogenic potential may be further augmented by genes encoding bone morphogenetic proteins (BMP’s). The purpose of this study was to compare the effect of genetic modification of human and equine BMDMSCs with BMP-2 or 7 or BMP-2 and 7 on their osteoblastogenic differentiation in the presence or absence of dexamethasone. The BMDMSCs were harvested from the iliac crest of 3 human donors and tuber coxae of 3 equine donors. Monolayer cells were genetically modified using adenovirus vectors encoding BMP-2, -7 or both and cultured in the presence or absence of dexamethasone. Expression of BMPs was confirmed by enzyme linked immunosorbent assay. To evaluate osteoblastic differentiation, cellular morphology was assessed every other day and expression and secretion of alkaline phosphatase (ALP), as well as expression levels of osteonectin, osteocalcin, and Runx2 were measured for up to 14 days. Human and equine BMDMSCs showed a capacity for osteogenic differentiation regardless of genetic modification or dexamethasone supplementation. Dexamethasone supplementation was more important for osteoblastogenic differentiation of equine BMDMSCs than human BMDMSCs. Genetic modification of BMDMSCs increased ALP secretion with AdBMP-2 homodimer having the greatest effect in both human and equine cells compared to AdBMP 7 or AdBMP 2/7. BMP protein elution rates reached their maximal concentration between day 4 and 8 and remained relatively stable thereafter, suggesting that genetically modified BMDMSCs could be useful for cell-based delivery of BMPs to a site of bone formation.
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DOI：
10.1002/jbm.820281005
发表时间：
1994-10-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
LEE, SC;SHEA, M;HAYES, WC
通讯作者：
HAYES, WC
影响因子：
2.6
作者：
LYONS, KM;HOGAN, BLM;ROBERTSON, EJ
通讯作者：
ROBERTSON, EJ
影响因子：
2.8
作者：
Kisiday, John D.;Kopesky, Paul W.;Frisbie, David D.
通讯作者：
Frisbie, David D.
影响因子：
2.7
作者：
Osyczka, AM;Diefenderfer, DL;Leboy, PS
通讯作者：
Leboy, PS
DOI：
10.1302/0301-620x.68b4.3733844
发表时间：
1986-01-01
影响因子：
--
作者：
NILSSON, OS;URIST, MR;FINERMAN, GAM
通讯作者：
FINERMAN, GAM