Synthetic chromosomes, genomes, viruses, and cells.
Synthetic chromosomes, genomes, viruses, and cells.
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DOI：
10.1016/j.cell.2022.06.046
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发表时间：
2022-07-21
期刊：
影响因子：
64.5
通讯作者：
Vashee, Sanjay
中科院分区：
文献类型：
--
作者：
Venter, J. Craig;Glass, John I.;Hutchison, Clyde A., III;Vashee, Sanjay
合成基因组学是用合成基因组的病毒，细菌和真核细胞的构建。它涉及两个基本过程：完整基因组或染色体的合成，并启动那些合成核酸以生成病毒或活细胞。第一个合成基因组学努力导致了病毒的构建。这导致了病毒反向遗传学的革命以及疫苗设计和制造的改善。具有合成基因组的第一个细菌导致了最小细菌细胞的构建，并重新编码了大肠杆菌菌株，能够在蛋白质中掺入多种非标准氨基酸，并且对噬菌体感染有抵抗力。进一步的进步导致酵母菌菌株具有合成基因组和动物和植物人造染色体的新方法。从地平洋上讲，DNA合成的进步将取得巨大的进步，这将使医学，工业，农业和研究方面具有非凡的新机会。
合成基因组学是合成生物学的一个分支，它用合成基因组构建病毒，细菌和真核细胞。 Venter及其同事回顾了合成基因组学的基础，进步，挑战和未来的前景。
Synthetic Genomics is the construction of viruses, bacteria, and eukaryotic cells with synthetic genomes. It involves two basic processes: synthesis of complete genomes or chromosomes and booting up of those synthetic nucleic acids to make viruses or living cells. The first Synthetic Genomics efforts resulted in the construction of viruses. This led to a revolution in viral reverse genetics and improvements in vaccine design and manufacture. The first bacterium with a synthetic genome led to construction of a minimal bacterial cell and recoded Escherichia coli strains able to incorporate multiple non-standard amino acids in proteins and resistant to phage infection. Further advances led to a yeast strain with a synthetic genome and new approaches for animal and plant artificial chromosomes. On the horizon there are dramatic advances in DNA synthesis that will enable extraordinary new opportunities in medicine, industry, agriculture, and research. Synthetic Genomics is a branch of synthetic biology that constructs viruses, bacteria and eukaryotic cells with synthetic genomes. Venter and colleagues review the foundations, advances, challenges and future prospects of Synthetic Genomics.
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影响因子：
14.9
作者：
Egeland RD;Southern EM
通讯作者：
Southern EM
影响因子：
17.1
作者：
Dormitzer, Philip R.;Suphaphiphat, Pirada;Venter, J. Craig
通讯作者：
Venter, J. Craig
影响因子：
3.7
作者：
Brown, David M.;Glass, John, I
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Glass, John, I
影响因子：
64.8
作者：
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通讯作者：
Boeke, Jef D.
DOI：
10.1126/science.1249252
发表时间：
2014-04-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
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通讯作者：
Chandrasegaran S