Electron probe analysis of calcium transport by small intestine.

Electron probe analysis of calcium transport by small intestine.
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DOI:
10.1083/jcb.64.1.54
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发表时间:
1975-01
影响因子:
7.8
通讯作者:
COLEMAN, JR
COLEMAN, JR
中科院分区:
生物学1区
文献类型:
--
作者:
WARNER, RR;COLEMAN, JR

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使用电子探针 X 射线微量分析仪在细胞水平上研究了大鼠和小鸡小肠中的钙转运。取出组织后以及在钙溶液中孵育后直接进行检查。在这两种制剂中,发现离散的钙定位与细胞内和细胞外杯状细胞粘液相关。体外制剂显示钙在不连续的位置上穿过吸收性上皮。尽管运输的主要路径是沿着细胞侧边界,但在细胞质的核上位置发现了一些定位。维生素 D 消耗和补充的作用是分别减少和增加穿过上皮细胞的钙定位数量。这些结果表明,钙以隔离形式运输,并表明杯状细胞粘液在此运输过程中发挥作用。
Calcium transport in small intestine of rat and chick has been studied at the cellular level using the electron probe X-ray microanalyzer. Tissues were examined directly after removal as well as after incubation in a calcium solution. In both preparations, discrete calcium localizations were found associated with intracellular and extracellular goblet cell mucus. The in vitro preparations showed calcium in transit across the absorptive epithelium in discrete localizations. Although the primary path of transport was along lateral cell borders, some localizations were found in the cytoplasm in a supranuclear position. The effect of vitamin D depletion and repletion was to decrease and increase, respectively, the number of calcium localizations in transit across the epithelium. These results suggest that calcium is transported while in a sequestered form and indicate that goblet cell mucus plays a role in this transport process.
DOI: 10.1083/jcb.45.3.635
发表时间: 1970-06
期刊: The Journal of cell biology
影响因子: --
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发表时间: 1965-01-01
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