Developmental exposure window influences silver toxicity but does not affect the susceptibility to subsequent exposures in zebrafish embryos.
Developmental exposure window influences silver toxicity but does not affect the susceptibility to subsequent exposures in zebrafish embryos.
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发育暴露窗口会影响银毒性，但不会影响斑马鱼胚胎中随后暴露的敏感性。
DOI：
10.1007/s00418-020-01933-2
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发表时间：
2020-11
影响因子：
2.3
通讯作者：
Santos EM
中科院分区：
文献类型：
--
作者：
Robinson PC;Littler HR;Lange A;Santos EM
银是一种非必需的有毒金属，广泛存在于淡水中，能够对野生动物造成不利影响。其毒性作用已被详细研究，但对发育过程中敏感性如何变化以及预暴露是否影响再次暴露后的耐受性知之甚少。我们使用斑马鱼胚胎（Danio rerio）模型来解决这些知识空白，以研究在囊胚中期过渡之前的发育阶段（当绒毛膜硬化和表观遗传重编程发生时）暴露是否比在此期间之后开始的暴露导致更大的毒性。我们在受精后0.5 h （hpf）和4 hpf开始接触银，以确定毒性是否不同。同时，我们将胚胎暴露于甲基化抑制剂5-氮杂胞苷中作为阳性对照。与4 hpf相比，暴露于0.5 hpf时毒性增加，银和5-氮杂胞苷的LC50分别显著降低1.2倍和7.6倍。然后，我们研究了在发育早期（从0.5或4 hpf）预先暴露于银是否会影响幼虫阶段后续暴露的结果，并发现与naïve幼虫相比，毒性没有变化。总之，这些数据表明，在发育早期，在单细胞阶段开始实验时，斑马鱼胚胎对银更敏感，但预暴露不影响后续暴露的结果，这表明在我们的实验条件下，没有能够影响未来敏感性的持久记忆。在单细胞阶段开始接触的毒性更大，这一发现对设计评估化学毒性的测试系统具有启示意义。本文的在线版本（10.1007/s00418-020-01933-2）包含补充资料，仅供授权用户使用。
Silver is a non-essential, toxic metal widespread in freshwaters and capable of causing adverse effects to wildlife. Its toxic effects have been studied in detail but less is known about how sensitivity varies during development and whether pre-exposures affect tolerance upon re-exposure. We address these knowledge gaps using the zebrafish embryo (Danio rerio) model to investigate whether exposures encompassing stages of development prior to mid-blastula transition, when chorion hardening and epigenetic reprogramming occur, result in greater toxicity compared to those initiated after this period. We conducted exposures to silver initiated at 0.5 h post fertilisation (hpf) and 4 hpf to determine if toxicity differed. In parallel, we exposed embryos to the methylation inhibitor 5-azacytidine as a positive control. Toxicity increased when exposures started from 0.5 hpf compared to 4 hpf and LC50 were significantly lower by 1.2 and 7.6 times for silver and 5-azacyitidine, respectively. We then investigated whether pre-exposure to silver during early development (from 0.5 or 4 hpf) affected the outcome of subsequent exposures during the larvae stage, and found no alterations in toxicity compared to naïve larvae. Together, these data demonstrate that during early development zebrafish embryos are more sensitive to silver when experiments are initiated at the one-cell stage, but that pre-exposures do not influence the outcome of subsequent exposures, suggesting that no long-lasting memory capable of influencing future susceptibility was maintained under our experimental conditions. The finding that toxicity is greater for exposures initiated at the one-cell stage has implications for designing testing systems to assess chemical toxicity. The online version of this article (10.1007/s00418-020-01933-2) contains supplementary material, which is available to authorized users.
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影响因子：
4.6
作者：
Dorts J;Falisse E;Schoofs E;Flamion E;Kestemont P;Silvestre F
通讯作者：
Silvestre F
DOI：
10.1016/j.btre.2016.07.001
发表时间：
2016-09
期刊：
Biotechnology reports (Amsterdam, Netherlands)
影响因子：
--
作者：
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通讯作者：
Mani, Vijay
影响因子：
3.8
作者：
Bury, NR;Grosell, M;Wood, CM
通讯作者：
Wood, CM
影响因子：
11.4
作者：
Ashauer, Roman;O'Connor, Isabel;Escher, Beate I.
通讯作者：
Escher, Beate I.
影响因子：
2.7
作者：
Coe, T. S.;Hamilton, P. B.;Tyler, C. R.
通讯作者：
Tyler, C. R.