DNA methyltransferases and stress-related genes expression in zebrafish larvae after exposure to heat and copper during reprogramming of DNA methylation.
DNA methyltransferases and stress-related genes expression in zebrafish larvae after exposure to heat and copper during reprogramming of DNA methylation.
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DOI：
10.1038/srep34254
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发表时间：
2016-10-12
影响因子：
4.6
通讯作者：
Silvestre F
中科院分区：
文献类型：
--
作者：
Dorts J;Falisse E;Schoofs E;Flamion E;Kestemont P;Silvestre F
DNA甲基化是一个被充分研究的表观遗传标记，对成年期和发育过程中的基因调控非常重要。利用遗传学和表观遗传学方法，本研究旨在评估热应激和铜暴露对斑马鱼早期胚胎发生的影响，此时DNA甲基化模式正在建立，这一过程被称为重编程。胚胎在26.5°C或34°C条件下暴露于325 μg Cu/L（受精后<1 h - hpf）至4 hpf，然后在26.5°C清水中孵育至96 hpf。在高温和铜联合暴露后，观察到死亡率显著增加和孵化延迟。其次，两种应激源单独或联合作用均显著上调新生DNA甲基转移酶基因（dnmt3）的表达，但总体胞嘧啶甲基化水平无差异。最后，Cu暴露显著增加了金属硫蛋白（metallothionein, mt2）和热休克蛋白（heat shock protein, hsp70）的表达，后者在高温暴露后也增加。这些结果强调了早期胚胎发生的敏感性，更准确地说，是重编程时期对环境挑战的敏感性，在一个现实的情况下，综合压力。
DNA methylation, a well-studied epigenetic mark, is important for gene regulation in adulthood and for development. Using genetic and epigenetic approaches, the present study aimed at evaluating the effects of heat stress and copper exposure during zebrafish early embryogenesis when patterns of DNA methylation are being established, a process called reprogramming. Embryos were exposed to 325 μg Cu/L from fertilization (<1 h post fertilization - hpf) to 4 hpf at either 26.5 °C or 34 °C, followed by incubation in clean water at 26.5 °C till 96 hpf. Significant increased mortality rates and delayed hatching were observed following exposure to combined high temperature and Cu. Secondly, both stressors, alone or in combination, significantly upregulated the expression of de novo DNA methyltransferase genes (dnmt3) along with no differences in global cytosine methylation level. Finally, Cu exposure significantly increased the expression of metallothionein (mt2) and heat shock protein (hsp70), the latter being also increased following exposure to high temperature. These results highlighted the sensitivity of early embryogenesis and more precisely of the reprogramming period to environmental challenges, in a realistic situation of combined stressors.
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影响因子：
3.6
作者：
Baccarelli A;Bollati V
通讯作者：
Bollati V
影响因子：
3.9
作者：
Bourdineaud, J. -P.;Baudrimont, M.;Moreau, J. -L.
通讯作者：
Moreau, J. -L.
DOI：
10.1016/s1095-6433(03)00033-3
发表时间：
2003-04-01
影响因子：
2.3
作者：
Airaksinen, S;Råbergh, CMI;Nikinmaa, M
通讯作者：
Nikinmaa, M
斑马鱼发育过程中的全球和基因特异性DNA甲基化变化。
DOI：
10.1016/j.cbpb.2013.07.007
发表时间：
2013-09
影响因子：
2.2
作者：
Fang, Xiefan;Corrales, Jone;Thornton, Cammi;Scheffler, Brian E.;Willett, Kristine L.
通讯作者：
Willett, Kristine L.
斑马鱼早期胚胎遗传了精子而非卵母细胞的DNA甲基化组
影响因子：
64.5
作者：
Jiang L;Zhang J;Wang JJ;Wang L;Zhang L;Li G;Yang X;Ma X;Sun X;Cai J;Zhang J;Huang X;Yu M;Wang X;Liu F;Wu CI;He C;Zhang B;Ci W;Liu J
通讯作者：
Liu J