In-channel amperometric detection for microchip electrophoresis using a wireless isolated potentiostat.
In-channel amperometric detection for microchip electrophoresis using a wireless isolated potentiostat.
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DOI：
10.1002/elps.201000681
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发表时间：
2011-04
期刊：
影响因子：
2.9
通讯作者：
Lunte, Susan M.
中科院分区：
文献类型：
--
作者：
Gunasekara, Dulan B.;Hulvey, Matthew K.;Lunte, Susan M.
关键词：
微芯片电泳 (ME) 与电流检测的结合带来了许多与将检测器与用于分离的高电压隔离相关的分析挑战。虽然已经采用了端通道对准和使用解耦器等方法，但它们有局限性。一种不太常见的方法是使用电隔离恒电位仪。这种方法允许将工作电极直接放置在分离通道中，而无需使用解耦器。本文探讨了使用微芯片电泳和电化学检测（ME-EC）与电隔离恒电位仪来分离和通道内检测几种重要的生物学阴离子。该分离采用负极性电压和十四烷基三甲基溴化铵（TTAB，作为缓冲液调节剂）来分离亚硝酸盐 (NO2-)、谷胱甘肽 (GSH)、抗坏血酸 (AA) 和酪氨酸 (Tyr)。与端通道相比，通道内配置中的 NO2- 和 H2O2 标准品的半波电位 (E½) 偏移约为负 500 mV。使用通道内检测配置观察到 NO2- 和 H2O2 具有更高的分离效率。与末端通道相比，通道内亚硝酸盐检测的检测限大约低两倍，灵敏度大约高两倍。描述了该微流体装置在分离和检测与氧化应激相关的生物标志物中的应用。
The combination of microchip electrophoresis (ME) with amperometric detection leads to a number of analytical challenges that are associated with isolating the detector from the high voltages used for the separation. While methods such as end-channel alignment and the use of decouplers have been employed, they have limitations. A less common method has been to utilize an electrically isolated potentiostat. This approach allows placement of the working electrode directly in the separation channel without using a decoupler. This paper explores the use of microchip electrophoresis and electrochemical detection (ME-EC) with an electrically isolated potentiostat for the separation and in-channel detection of several biologically important anions. The separation employed negative polarity voltages and tetradecyltrimethylammonium bromide (TTAB, as a buffer modifier) for the separation of nitrite (NO2-), glutathione (GSH), ascorbic acid (AA), and tyrosine (Tyr). A half-wave potential (E½) shift of approximately negative 500 mV was observed for NO2- and H2O2 standards in the in-channel configuration compared to end channel. Higher separation efficiencies were observed for both NO2- and H2O2 with the in-channel detection configuration. The limits of detection were approximately two-fold lower and the sensitivity was approximately two-fold higher for in-channel detection of nitrite when compared to end-channel. The application of this microfluidic device for the separation and detection of biomarkers related to oxidative stress is described.
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