Changes in antioxidant activity of Alcalase-hydrolyzed soybean hydrolysate under simulated gastrointestinal digestion and transepithelial transport
Changes in antioxidant activity of Alcalase-hydrolyzed soybean hydrolysate under simulated gastrointestinal digestion and transepithelial transport
复制标题
Alcalase水解大豆水解物在模拟胃肠消化和跨上皮运输下抗氧化活性的变化
DOI：
10.1016/j.jff.2018.01.017
复制
发表时间：
2018-03
影响因子：
5.6
通讯作者：
Lianzhou Jiang
中科院分区：
文献类型：
--
作者：
Qiaozhi Zhang;Xiaohong Tong;Baokun Qi;Zhongjiang Wang;Yang Li;Xiaonan Sui;Lianzhou Jiang
碱解大豆蛋白水解物(SPH)中的多肽可能具有作为天然抗氧化剂的潜力。此外，人类胃肠道(GI)对多肽生物利用度的影响还有待探讨。在这项研究中，模拟胃肠道消化和跨皮转运对SPH的各种抗氧化性能的影响进行了研究。SPH具有清除DPPH自由基(IC50=4.22 mg/mL)、清除ABTS%+自由基(IC50=2.93 mg/mL)、还原能力和金属离子螯合活性(IC50=0.67 mg/mL)。此外，SPH显著抑制Caco-2细胞内ROS的产生(P<；0.05)。模拟胃肠消化后，SPH的抗氧化性能有所提高，但ABTS%+自由基清除活性降低。在跨皮转运后，渗透液保持了部分抗氧化活性，LC-MS/MS数据进一步鉴定了吸附的大豆肽。这些结果表明，SPH含有潜在的生物可利用的抗氧化肽，可以被认为是一种有前途的功能性食品成分来源。
Peptides from Alcalase-hydrolyzed soybean protein hydrolysate (SPH) may hold the potential as natural antioxidants. In addition, the effect of human gastrointestinal (GI) tract on peptide bioavailability needs to be explored. In this study, the impact of simulated GI digestion and transepithelial transport on various antioxidant properties of SPH were investigated. SPH displayed DPPH radical scavenging (IC50 = 4.22 mg/mL), ABTS%+ radical scavenging (IC50 = 2.93 mg/mL), reducing power and metal ion-chelating activities (IC50 = 0.67 mg/ mL). Furthermore, SPH significantly (P < .05) inhibited the generation of intracellular reactive oxygen species (ROS) in Caco-2 cells. After simulated GI digestion, the antioxidant properties of SPH were enhanced, except for a decrease in ABTS%+ radical scavenging activity. After transepithelial transport, the permeates maintained partial antioxidant activity and the LC-MS/MS data further identified the absorbed soybean peptides. These results suggest that SPH contains the antioxidant peptides that are potentially bioavailable and can be regarded as a promising source of functional food ingredients.
登录
查看更多内容
影响因子：
8.8
作者：
You, Lijun;Zhao, Mouming;Ren, Jiaoyan
通讯作者：
Ren, Jiaoyan
影响因子：
8.8
作者：
Ketnawa, Sunantha;Martinez-Alvarez, Oscar;Rawdkuen, Saroat
通讯作者：
Rawdkuen, Saroat
影响因子：
8.1
作者：
Cian, Raul E.;Martinez-Augustin, Olga;Drago, Silvina R.
通讯作者：
Drago, Silvina R.
影响因子：
3
作者：
Chen, Ning;Yang, Hongmei;Liu, Shuying
通讯作者：
Liu, Shuying
影响因子：
6.1
作者：
Zhu, Lijuan;Chen, Jie;Xiong, Youling L.
通讯作者：
Xiong, Youling L.