Circulating mucosal associated invariant T cells are activated in Vibrio cholerae O1 infection and associated with lipopolysaccharide antibody responses.
Circulating mucosal associated invariant T cells are activated in Vibrio cholerae O1 infection and associated with lipopolysaccharide antibody responses.
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DOI：
10.1371/journal.pntd.0003076
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发表时间：
2014-08
影响因子：
3.8
通讯作者：
Ryan ET
中科院分区：
文献类型：
--
作者：
Leung DT;Bhuiyan TR;Nishat NS;Hoq MR;Aktar A;Rahman MA;Uddin T;Khan AI;Chowdhury F;Charles RC;Harris JB;Calderwood SB;Qadri F;Ryan ET
Mucosal Associated Invariant T (MAIT) cells are innate-like T cells found in abundance in the intestinal mucosa, and are thought to play a role in bridging the innate-adaptive interface. We measured MAIT cell frequencies and antibody responses in blood from patients presenting with culture-confirmed severe cholera to a hospital in Dhaka, Bangladesh at days 2, 7, 30, and 90 of illness. We found that MAIT (CD3+CD4−CD161hiVα7.2+) cells were maximally activated at day 7 after onset of cholera. In adult patients, MAIT frequencies did not change over time, whereas in child patients, MAITs were significantly decreased at day 7, and this decrease persisted to day 90. Fold changes in MAIT frequency correlated with increases in LPS IgA and IgG, but not LPS IgM nor antibody responses to cholera toxin B subunit. In the acute phase of cholera, MAIT cells are activated, depleted from the periphery, and as part of the innate response against V. cholerae infection, are possibly involved in mechanisms underlying class switching of antibody responses to T cell-independent antigens. Vibrio cholerae is the bacterium that causes cholera, which can be a potentially fatal diarrheal disease that affects millions of people worldwide each year. How our immune system provides protection against cholera is poorly understood. Mucosal Associated Invariant T (MAIT) cells are recently discovered immune cells found in the blood and intestinal tract of humans. In this study of cholera patients in Dhaka, Bangladesh, we found that blood MAIT cells are activated during cholera, and that in children, blood MAIT cells are decreased in number during the course of disease. We also found that the MAIT cell response correlates with the antibody response to V. cholerae O1 lipopolysaccharide, which in the past has been shown to be an important determinant of protection. These findings suggest that MAIT cells may play an important role in the body's defense against cholera.
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影响因子：
30.5
作者：
Le Bourhis, Lionel;Martin, Emmanuel;Lantz, Olivier
通讯作者：
Lantz, Olivier
影响因子：
9.8
作者：
Martin E;Treiner E;Duban L;Guerri L;Laude H;Toly C;Premel V;Devys A;Moura IC;Tilloy F;Cherif S;Vera G;Latour S;Soudais C;Lantz O
通讯作者：
Lantz O
影响因子：
6.7
作者：
Le Bourhis L;Dusseaux M;Bohineust A;Bessoles S;Martin E;Premel V;Coré M;Sleurs D;Serriari NE;Treiner E;Hivroz C;Sansonetti P;Gougeon ML;Soudais C;Lantz O
通讯作者：
Lantz O
影响因子：
3.4
作者：
Berkey CD;Blow N;Watnick PI
通讯作者：
Watnick PI
影响因子：
--
作者：
Johnson, Russell A.;Uddin, Taher;Ryan, Edward T.
通讯作者：
Ryan, Edward T.