Ca14-3-3 Interacts With CaWRKY58 to Positively Modulate Pepper Response to Low-Phosphorus Starvation.
Ca14-3-3 Interacts With CaWRKY58 to Positively Modulate Pepper Response to Low-Phosphorus Starvation.
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Ca14-3-3 与 CaWRKY58 相互作用，积极调节辣椒对低磷饥饿的反应
DOI：
10.3389/fpls.2020.607878
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发表时间：
2020
影响因子：
5.6
通讯作者：
He S
中科院分区：
文献类型：
--
作者：
Cai J;Cai W;Huang X;Yang S;Wen J;Xia X;Yang F;Shi Y;Guan D;He S
低磷胁迫和病原菌侵袭是植物在自然环境中经常经历的两种重要胁迫，但植物如何协调响应这两种胁迫的研究还不多见。在这里，我们证明了CaWRKY58，一个已知的辣椒(Capsicum Annuum)对青枯菌攻击反应的负调控因子，被内毒素上调。病毒诱导的基因沉默(VIGS)和CaWRKY58在烟草植株中的过表达，结合染色质免疫沉淀(ChIP)和凝胶迁移率改变分析(EMSA)表明，CaWRKY58通过直接靶向和调控与耐低磷相关基因，包括磷酸盐饥饿反应1(Phr1)，正向调节辣椒对LPS的响应。酵母双杂交实验表明，CaWRKY58与一个14-3-3蛋白(Ca14-3-3)相互作用；这种相互作用被下拉、双分子荧光互补(BIFC)和微量热电泳法(MST)证实。Ca14-3-3和CaWRKY58的相互作用促进了CaWRKY58对Phr1表达的激活，但不影响CaWRKY58负调控的免疫相关基因CaNPR1和CaDEF1的表达。综上所述，我们的数据表明，CaWRKY58负向调节对青枯雷尔斯氏菌的免疫，但正向调节对内毒素的耐受性，Ca14-3-3转录激活CaWRKY58以响应内毒素。
Low-phosphorus stress (LPS) and pathogen attack are two important stresses frequently experienced by plants in their natural habitats, but how plant respond to them coordinately remains under-investigated. Here, we demonstrate that CaWRKY58, a known negative regulator of the pepper (Capsicum annuum) response to attack by Ralstonia solanacearum, is upregulated by LPS. Virus-induced gene silencing (VIGS) and overexpression of CaWRKY58 in Nicotiana benthamiana plants in combination with chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assays (EMSA) demonstrated that CaWRKY58 positively regulates the response of pepper to LPS by directly targeting and regulating genes related to phosphorus-deficiency tolerance, including PHOSPHATE STARVATION RESPONSE1 (PHR1). Yeast two-hybrid assays revealed that CaWRKY58 interacts with a 14-3-3 protein (Ca14-3-3); this interaction was confirmed by pull-down, bimolecular fluorescence complementation (BiFC), and microscale thermophoresis (MST) assays. The interaction between Ca14-3-3 and CaWRKY58 enhanced the activation of PHR1 expression by CaWRKY58, but did not affect the expression of the immunity-related genes CaNPR1 and CaDEF1, which are negatively regulated by CaWRKY58 in pepper upon Ralstonia solanacearum inoculation. Collectively, our data indicate that CaWRKY58 negatively regulates immunity against Ralstonia solanacearum, but positively regulates tolerance to LPS and that Ca14-3-3 transcriptionally activates CaWRKY58 in response to LPS.
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影响因子：
6.9
作者：
Encinas-Villarejo, Sonia;Maldonado, Ana M.;Caballero, Jose L.
通讯作者：
Caballero, Jose L.
影响因子：
9.8
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Xie JK
影响因子：
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作者：
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通讯作者：
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影响因子：
3.7
作者：
Fan F;Cui B;Zhang T;Qiao G;Ding G;Wen X
通讯作者：
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