Transcriptome analysis of phosphorus stress responsiveness in the seedlings of Dongxiang wild rice (Oryza rufipogon Griff.).
Transcriptome analysis of phosphorus stress responsiveness in the seedlings of Dongxiang wild rice (Oryza rufipogon Griff.).
复制标题
东乡野生稻幼苗磷胁迫反应的转录组分析
DOI：
10.1186/s40659-018-0155-x
复制
发表时间：
2018-03-15
影响因子：
6.7
通讯作者：
Xie JK
中科院分区：
文献类型：
--
作者：
Deng QW;Luo XD;Chen YL;Zhou Y;Zhang FT;Hu BL;Xie JK
【摘要】：背景:磷利用率低是制约水稻生长的主要因素。东乡野生稻(Oryza rufipogon Griff.)具有栽培稻所缺乏的许多有用基因，包括抗缺磷、耐寒、耐盐、耐旱等逆境，被认为是水稻育种的宝贵种质资源。然而，耐缺磷调控的分子机制尚不清楚。结果：本研究以磷胁迫和未处理的东乡野生稻幼苗的叶和根组织为样本构建cDNA文库，并进行转录组测序，以阐明磷胁迫响应的分子机制。结果表明，1184个转录本在叶中差异表达（323个上调，861个下调），986个转录本在根中差异表达（756个上调，230个下调）。 43个基因在叶和根中均上调，38个基因在根中上调但在叶片中下调，只有2个基因在根中下调但在叶片中上调。在这些差异表达基因中，许多转录因子和功能基因的检测表明，缺磷耐受性涉及多种调控途径。同时，还通过功能分类和京都基因百科全书和基因组通路图谱分别对差异表达基因进行了基因本体术语和关键通路注释。将本研究发现的差异表达基因与前期鉴定的耐缺磷数量性状位点相结合，筛选出一组最重要的候选基因。结论：本工作为东乡野生稻耐缺磷性的功能解析提供了丰富的基因组信息，有助于了解东乡野生稻耐缺磷的生物调控机制。 东乡茭白。
Background:Low phosphorus availability is a major factor restricting rice growth. Dongxiang wild rice (Oryza rufipogon Griff.) has many useful genes lacking in cultivated rice, including stress resistance to phosphorus deficiency, cold, salt and drought, which is considered to be a precious germplasm resource for rice breeding. However, the molecular mechanism of regulation of phosphorus deficiency tolerance is not clear.Results:In this study, cDNA libraries were constructed from the leaf and root tissues of phosphorus stressed and untreated Dongxiang wild rice seedlings, and transcriptome sequencing was performed with the goal of elucidating the molecular mechanisms involved in phosphorus stress response. The results indicated that 1184 transcripts were differentially expressed in the leaves (323 up-regulated and 861 down-regulated) and 986 transcripts were differentially expressed in the roots (756 up-regulated and 230 down-regulated). 43 genes were up-regulated both in leaves and roots, 38 genes were up-regulated in roots but down-regulated in leaves, and only 2 genes were down-regulated in roots but up-regulated in leaves. Among these differentially expressed genes, the detection of many transcription factors and functional genes demonstrated that multiple regulatory pathways were involved in phosphorus deficiency tolerance. Meanwhile, the differentially expressed genes were also annotated with gene ontology terms and key pathways via functional classification and Kyoto Encyclopedia of Gene and Genomes pathway mapping, respectively. A set of the most important candidate genes was then identified by combining the differentially expressed genes found in the present study with previously identified phosphorus deficiency tolerance quantitative trait loci.Conclusion:The present work provides abundant genomic information for functional dissection of the phosphorus deficiency resistance of Dongxiang wild rice, which will be help to understand the biological regulatory mechanisms of phosphorus deficiency tolerance in Dongxiang wild rice.
登录
查看更多内容
DOI：
10.1002/anie.201705010
发表时间：
2017-08-01
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Dang TT;Franke J;Tatsis E;O'Connor SE
通讯作者：
O'Connor SE
影响因子：
7.4
作者：
Chin, Joong Hyoun;Gamuyao, Rico;Heuer, Sigrid
通讯作者：
Heuer, Sigrid
影响因子：
9.4
作者：
Guo, B.;Jin, Y.;Versaw, Wayne K.
通讯作者：
Versaw, Wayne K.
影响因子：
64.8
作者：
Huang X;Kurata N;Wei X;Wang ZX;Wang A;Zhao Q;Zhao Y;Liu K;Lu H;Li W;Guo Y;Lu Y;Zhou C;Fan D;Weng Q;Zhu C;Huang T;Zhang L;Wang Y;Feng L;Furuumi H;Kubo T;Miyabayashi T;Yuan X;Xu Q;Dong G;Zhan Q;Li C;Fujiyama A;Toyoda A;Lu T;Feng Q;Qian Q;Li J;Han B
通讯作者：
Han B
影响因子：
2.8
作者：
Lee, Jeongyeo;Han, Ching-Tack;Hur, Yoonkang
通讯作者：
Hur, Yoonkang