Low expression of LOC285194 is associated with poor prognosis in colorectal cancer.
Low expression of LOC285194 is associated with poor prognosis in colorectal cancer.
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DOI：
10.1186/1479-5876-11-122
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发表时间：
2013-05-16
影响因子：
7.4
通讯作者：
Du X
中科院分区：
文献类型：
--
作者：
Qi P;Xu MD;Ni SJ;Huang D;Wei P;Tan C;Zhou XY;Du X
长链非编码RNA（lncRNA）的研究已逐渐成为RNA生物学领域的热点之一。L0 C285194是一种在骨肉瘤中具有潜在抑瘤作用的lncRNA。本研究的目的是检测结直肠癌（CRC）患者中LOC 285194的表达，并研究该lncRNA水平与现有临床病理参数和患者生存率之间的关系。采用实时荧光定量聚合酶链反应检测81例随访5年的结直肠癌患者癌组织和癌旁正常组织、3株结直肠癌细胞系和正常肠黏膜细胞系中LOC 285194的表达。然后，我们分析了这种lncRNA在肿瘤组织中的水平与结直肠癌的现有临床病理特征和临床结局之间的潜在关系。LOC 285194在大肠癌组织和大肠癌细胞系中的相对表达水平显著低于癌旁正常组织和正常肠粘膜细胞系（p < 0.001）。此外，LOC 285194的低表达与较大的肿瘤大小（p = 0.015）、较高的肿瘤分期（p = 0.034）和较多的远处转移（p = 0.046）相关。Kaplan-Meier分析表明，具有低LOC 285194表达的患者具有较差的无病生存期（p = 0.010）。此外，多变量分析表明，LOC 285194的表达减少是疾病特异性生存的独立预测因素。我们的数据表明，LOC 285194可能是一个新的预后指标，在大肠癌的诊断和基因治疗可能是一个潜在的目标。
The long non-coding RNAs (lncRNAs) study has gradually become one of the hot topics in the field of RNA biology. One lncRNA which has attracted attention is LOC285194, a lncRNA demonstrated the potential tumor-suppressor role in osteosarcoma. The aim of this study was to examine the expression of LOC285194 in colorectal cancer (CRC) patients and to investigate the relationship between this lncRNA levels and existing clinicopathologic parameters and patient survival. The expression of LOC285194 was detected by quantitative real-time polymerase chain reaction in pairs of tumorous and adjacent normal tissues of 81 colorectal cancer patients with a follow-up of 5 years, as well as in three colorectal cancer cell lines and normal intestinal mucous cell line. Then, we analyzed the potential relationship between this lncRNA levels in tumor tissues and existing clinicopathological features of CRC, and clinical outcome. The relative expression levels of LOC285194 was significantly lower in tumor tissues (p < 0.001) and colorectal cancer cell lines compared with adjacent normal tissues and normal intestinal mucous cell line. In addition, low expression of LOC285194 was correlated with larger tumor size (p = 0.015), higher tumor stage (p = 0.034), and more distant metastasis (p = 0.046). Kaplan-Meier analysis indicated that patients with low LOC285194 expression had a poor disease free survival (p = 0.010). Moreover, multivariate analysis showed that decreased expression of LOC285194 was an independent predictor of disease-specific survival. Our data indicate that LOC285194 might be a novel prognostic indicator in colorectal cancer and may be a potential target for diagnosis and gene therapy.
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影响因子：
37.3
作者：
Gibb EA;Brown CJ;Lam WL
通讯作者：
Lam WL
影响因子：
64.5
作者：
Huarte M;Guttman M;Feldser D;Garber M;Koziol MJ;Kenzelmann-Broz D;Khalil AM;Zuk O;Amit I;Rabani M;Attardi LD;Regev A;Lander ES;Jacks T;Rinn JL
通讯作者：
Rinn JL
影响因子：
3.7
作者：
Reis EM;Verjovski-Almeida S
通讯作者：
Verjovski-Almeida S
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
8
作者：
Lin, R.;Maeda, S.;Edgington, T. S.
通讯作者：
Edgington, T. S.