Quantitative phase imaging to improve the diagnostic accuracy of urine cytology.
Quantitative phase imaging to improve the diagnostic accuracy of urine cytology.
复制标题
DOI：
10.1002/cncy.21734
复制
发表时间：
2016-09
影响因子：
3.4
通讯作者：
Liu, Yang
中科院分区：
文献类型：
--
作者：
Pham, Hoa V.;Pantanowitz, Liron;Liu, Yang
尿液细胞学确诊为尿路上皮癌通常具有挑战性和主观性。几个尿细胞学样本被诊断为“不确定”。荧光原位杂交(FISH)等辅助技术已被用来试图提高诊断的敏感性，但这并未被批准作为常规筛查试验，而且复杂的荧光染色方案也限制了其广泛的临床应用。定量相位成像(QPI)是一种能够精确测量单细胞干质量的新兴技术。本研究旨在探讨定量时相成像技术在提高尿液细胞学恶性诊断准确性中的作用。我们对28例四种细胞学诊断(阴性、不典型、可疑和恶性阳性)的未染色的ThinPrep准备的尿细胞学切片进行了定量时相成像。计算每个患者数百个细胞的核/细胞干质量、它们的熵和核/细胞质量比，然后将其与后续诊断相关联。尿路上皮细胞的核质量及其熵在阴性组和阳性组之间差异有统计学意义。这些数据显示从阴性到不典型/可疑和阳性细胞学诊断的患者逐渐增加。最重要的是，对于那些不典型或可疑组的患者，那些有恶性肿瘤随访诊断的患者的核质量及其熵显著高于那些没有后续恶性随访的患者。定量时相成像显示了提高尿细胞学诊断准确率的潜力，特别是对于不确定的病例，应进一步评估其作为尿细胞学辅助检查的价值。
Definitive diagnosis of urothelial carcinoma in urine cytology is often challenging and subjective. Several urine cytology samples receive an “indeterminate” diagnosis. Ancillary techniques such as fluorescence in situ hybridization (FISH) have been employed to try improve diagnostic sensitivity, but this is not approved as a routine screening test and the complex fluorescent staining protocol also limits its widespread clinical use. Quantitative phase imaging (QPI) is an emerging technology allowing accurate measurement of single-cell dry mass. This study was undertaken to explore the ability of quantitative phase imaging to improve the diagnostic accuracy of malignancy in urine cytology. We performed quantitative phase imaging on unstained ThinPrep-prepared urine cytology slides from 28 patients with four categories of cytological diagnosis (negative, atypical, suspicious and positive for malignancy). Nuclear/cell dry mass, their entropy and nucleus-to-cell mass ratio were calculated for several hundred cells for each patient, and were then correlated with follow-up diagnoses. The nuclear mass and its entropy of urothelial cells showed significant difference between negative and positive groups. These data showed progressive increase from patients with negative to atypical/suspicious and positive cytologic diagnoses. Most importantly, for those patients in atypical or suspicious groups, the nuclear mass and its entropy from those patients with a follow-up diagnosis of malignancy were significantly higher than those patients without a subsequent malignant follow-up. Quantitative phase imaging shows potential to improve the diagnostic accuracy of urine cytology, especially for indeterminate cases and should be further evaluated as an ancillary test for urine cytology.
登录
查看更多内容
影响因子：
6.6
作者：
Halling, KC;King, W;Jenkins, RB
通讯作者：
Jenkins, RB
影响因子：
3.6
作者：
Nguyen, T. H.;Edwards, C.;Popescu, G.
通讯作者：
Popescu, G.
影响因子：
3.6
作者：
Ou X;Horstmeyer R;Yang C;Zheng G
通讯作者：
Zheng G
影响因子：
6.6
作者：
Skacel, M;Fahmy, M;Tubbs, RR
通讯作者：
Tubbs, RR
影响因子：
3.5
作者：
Rappaz, Benjamin;Cano, Elena;Marquet, Pierre
通讯作者：
Marquet, Pierre