Some structural and functional aspects of the mitotic apparatus in sea urchin embryos.
Some structural and functional aspects of the mitotic apparatus in sea urchin embryos.
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DOI：
10.1083/jcb.14.3.475
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发表时间：
1962-09
影响因子：
7.8
通讯作者：
HARRIS, P
中科院分区：
文献类型：
--
作者：
HARRIS, P
有丝分裂的数字在海胆胚胎的分裂细胞，从第一次分裂的纤毛形成的发病，进行了研究方面的细丝系统和它的关系动粒，染色体，和极点，以及固定条件，这将最好地保存这些结构。关于固定，固定剂的盐浓度和pH值的变化表明，在早期的工作中注意到的染色体上的提取效果可能是由于中性pH值和相当于海水的盐浓度的组合。15 mµ细丝的存在取决于两种稳定条件中的任何一种：pH 6.1或海水盐的存在，可能是Ca和Mg的二价阳离子。动粒和中心粒不受固定剂变化的影响。早期在纺锤体中发现的15 mµ细丝，在早期卵裂分裂的紫苑中也发现了大量。然而，随着细胞的连续分裂和缩小，星形细胞在32至64个细胞阶段消失，15 mµ的细丝完全与中央纺锤体相连。紫菀的消失表明，事实上，它可能只是大细胞胞质分裂的专门化。
The mitotic figures in dividing cells of sea urchin embryos, from first division to the onset of cilia formation, were studied with regard to the filament system and its relation to kinetochores, chromosomes, and poles, as well as to fixation conditions which would best preserve these structures. With regard to fixation, variations in the salt concentration and pH of the fixative indicated that an extraction effect on the chromosomes noted in earlier work was probably due to a combination of neutral pH and salt concentration equivalent to sea water. The presence of the 15 mµ filaments depended on the presence of either of two stabilizing conditions: pH 6.1 or presence of the salts of sea water, presumably the divalent cations of Ca and Mg. Kinetochores and centrioles were unaffected by the fixative variations. The 15 mµ filaments, reported earlier in the central spindle, are also found in great numbers in the asters of early cleavage divisions. However, with successive divisions and reduction in cell size, the aster disappears at about the 32 to 64 cell stage, and the 15 mµ filaments are entirely associated with the central spindle. This disappearance of the aster suggests that it may be, in fact, merely a specialization of large cells for cytokinesis.
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DOI：
10.1083/jcb.12.1.57
发表时间：
1962-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
ROTH LE;DANIELS EW
通讯作者：
DANIELS EW
DOI：
10.1083/jcb.12.1.47
发表时间：
1962-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
KANE RE
通讯作者：
KANE RE
DOI：
10.1083/jcb.8.1.207
发表时间：
1960-01-01
期刊：
JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY
影响因子：
--
作者：
ROTH, LE;OBETZ, SW;DANIELS, EW
通讯作者：
DANIELS, EW
DOI：
10.1083/jcb.10.4.467
发表时间：
1961-08
期刊：
The Journal of biophysical and biochemical cytology
影响因子：
--
作者：
MAZIA D;MITCHISON JM;MEDINA H;HARRIS P
通讯作者：
HARRIS P
DOI：
10.1083/jcb.11.1.179
发表时间：
1961-10
期刊：
The Journal of biophysical and biochemical cytology
影响因子：
--
作者：
GIBBONS IR
通讯作者：
GIBBONS IR