Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture.
Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture.
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DOI：
10.1186/1471-2180-6-16
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发表时间：
2006-02-24
期刊：
影响因子：
4.2
通讯作者：
Kwang J
中科院分区：
文献类型：
--
作者：
Nwe N;He Q;Damrongwatanapokin S;Du Q;Manopo I;Limlamthong Y;Fenner BJ;Spencer L;Kwang J
预防可能发生的禽流感大流行需要开发快速诊断测试并最终生产疫苗。为生产疫苗，用重组杆状病毒表达H5N1禽流感的血凝素（HA1）。通过感染重组杆状病毒在单层或悬浮培养的昆虫细胞中表达重组HA1。悬浮培养的rHA1产量为68 mg/l，而单层培养的产量为6 mg/l。用50 μg的rHA1蛋白免疫豚鼠，两次接种后血凝素抑制和病毒中和效价为1:160。因此，利用昆虫悬浮细胞系统生产rHA1为经济生产H5抗原提供了有希望的基础。
Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine. For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein. Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.
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