Immunoglobulin heavy chain diversity in Pteropid bats: evidence for a diverse and highly specific antigen binding repertoire.
Immunoglobulin heavy chain diversity in Pteropid bats: evidence for a diverse and highly specific antigen binding repertoire.
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DOI：
10.1007/s00251-010-0425-4
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发表时间：
2010-03
期刊：
影响因子：
3.2
通讯作者：
Wang, Lin-Fa
中科院分区：
文献类型：
--
作者：
Baker, Michelle L.;Tachedjian, Mary;Wang, Lin-Fa
蝙蝠是一系列新出现和重新出现的病毒的天然宿主，其中许多病毒在其他哺乳动物中引起显著的发病率和死亡率，但似乎对蝙蝠没有临床后果。蝙蝠与多种病毒共存的能力提出了一个有趣的免疫学问题，尚未进行任何详细研究，但可以为哺乳动物抗病毒机制的进化提供重要见解。为了更好地了解蝙蝠的免疫系统，我们分析了表达的重链可变（VH）区的抗体从黑狐蝠，狐蝠。亲缘关系密切的翼蝠，Pteropus vampyrus，其基因组已被测序的生殖细胞库也进行了比较研究。在表达的P.alecto VH库和种系P.vampyrus VH库中的所有三种哺乳动物VH家族（I、II和III）中发现了代表性VH基因。使用多重重链多样性（DH）和连接（JH）区段来产生多样性VDJ重排的证据也存在于所表达的P.alecto抗体库中。蝙蝠与病毒的长期共同进化历史可能导致各种高度特异性的VH片段被硬连接到蝙蝠的基因组中，并可能影响它们成功科普多种病毒抗原的能力。
Bats are the natural host reservoir for range of emerging and re-emerging viruses, many of which cause significant morbidity and mortality in other mammals, yet appear to result in no clinical consequences for bats. The ability of bats to coexist with a variety of viruses presents an interesting immunological problem that has not been examined in any detail but which could provide significant insights into the evolution of antiviral mechanisms in mammals. Towards a better understanding of the bat immune system, we analysed the expressed heavy chain variable (VH) regions of antibodies from the black flying fox, Pteropus alecto. The germline repertoire of the closely related Pteropid bat, Pteropus vampyrus, whose genome has been sequenced was also examined for comparative purposes. Representative VH genes were found in all three mammalian VH clans (I, II and III) in both the expressed P. alecto VH repertoire and the germline P. vampyrus VH repertoire. Evidence for the use of multiple heavy chain diversity (DH) and joining (JH) segments for the generation of diverse VDJ rearrangements was also present in the expressed antibody repertoire of P. alecto. The long period of co-evolutionary history of bats with viruses may have resulted in a variety of highly specific VH segments being hardwired into the genomes of bats and may have implications for their ability to successfully cope with a diversity of viral antigens.
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