Cancer stemness of CD10-positive cells regulated by Hedgehog pathway promotes the resistance to cisplatin in oral squamous cell carcinoma.
Cancer stemness of CD10-positive cells regulated by Hedgehog pathway promotes the resistance to cisplatin in oral squamous cell carcinoma.
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Hedgehog通路调控的CD10阳性细胞的癌症干性促进口腔鳞状细胞癌对顺铂的耐药性。
DOI：
10.1111/odi.13673
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发表时间：
2020-10
期刊：
影响因子：
3.8
通讯作者：
Chuanbin Guo
中科院分区：
文献类型：
--
作者：
Yifei Wang;Qingxiang Li;Le Xu;Junpeng Chen;Yinfei Pu;Lin Wang;Hongfang Sun;Yuxing Guo;Chuanbin Guo
目的：探讨CD 10在口腔鳞状细胞癌（OSCC）顺铂耐药中的作用及其与Hedgehog（Hh）信号通路和肿瘤干细胞（CSCs）的关系。方法：分析顺铂处理后不同的口腔鳞癌细胞系中细胞存活率和CD 10表达之间的相关性。通过qPCR检测CD 10 high和CD 10 low OSCC细胞中与化疗耐药、肿瘤干细胞和上皮-间质转化相关的基因。采用小鼠移植瘤模型和静脉转移模型探讨CD 10对顺铂耐药的可能调控机制。结果：CD 10基因在不同细胞系中的高表达显示顺铂耐药能力增强。与CD 10 low细胞相比，CD 10 high细胞中与化疗抗性、细胞干细胞性和上皮-间质转化相关的基因表达显著更高。此外，顺铂和Hh通路抑制剂的组合显著降低了异种移植模型和静脉转移模型中CD 10对顺铂的抗性。结论：CD 10阳性细胞与口腔鳞状细胞癌顺铂耐药的发生有关，这可能与Hedgehog通路调控的肿瘤干细胞特性有关。
OBJECTIVE.To explore the role of CD10 in cisplatin-resistance of oral squamous cell carcinoma (OSCC) and its association with the Hedgehog (Hh) signaling pathway and cancer stem cells (CSCs)..METHODS.The correlation between cell viability and CD10 expression was analyzed in different OSCC cell lines after the cisplatin treatment. Genes related to chemotherapy-resistance, cancer stem cells, and the epithelial-mesenchymal transition were detected by qPCR in CD10high and CD10low OSCC cells. Mouse xenograft model and venous metastasis model were used to explore the potential regulatory mechanism of the resistance effect of CD10 on cisplatin..RESULTS.The higher expression of CD10 gene in different cell lines displayed enhanced cisplatin-resistance ability. The expression of genes related to chemotherapy-resistance, cell stemness, and the epithelial-mesenchymal transition was significantly higher in CD10high cells compared with CD10low cells. Moreover, the combination of cisplatin and Hh pathway inhibitors significantly reduced the resistance of CD10 to cisplatin in the xenograft model and venous metastasis models..CONCLUSION.CD10 positive cells are implicated in developing cisplatin-resistance of OSCC, which could be related to its cancer stem cell characteristics regulated by the Hedgehog pathway.
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DOI：
--
发表时间：
2018-11
期刊：
Journal of B.U.ON. : official journal of the Balkan Union of Oncology
影响因子：
--
作者：
M. Lazarević;Maja S. Milošević;D. Trišić;Boško Toljić;Jelena Simonović;N. Nikolic;Nikola Mikovic
通讯作者：
M. Lazarević;Maja S. Milošević;D. Trišić;Boško Toljić;Jelena Simonović;N. Nikolic;Nikola Mikovic
影响因子：
5.2
作者：
H. Kajiyama;K. Shibata;Mikio Terauchi;Mamoru Yamashita;K. Ino;A. Nawa;F. Kikkawa
通讯作者：
H. Kajiyama;K. Shibata;Mikio Terauchi;Mamoru Yamashita;K. Ino;A. Nawa;F. Kikkawa
头颈鳞状细胞癌的癌症干细胞生物标志物：生物信息分析
影响因子：
4.2
作者：
Xiao, Meng;Liu, Liu;Wang, Yan'an
通讯作者：
Wang, Yan'an
EMT，癌症干细胞和耐药性：癌症战争中新兴的邪恶轴。
影响因子：
8
作者：
Singh, A.;Settleman, J.
通讯作者：
Settleman, J.
影响因子：
64.8
作者：
Vasan N;Baselga J;Hyman DM
通讯作者：
Hyman DM