In vitro study on the feasibility of magnetic stent hyperthermia for the treatment of cardiovascular restenosis.
In vitro study on the feasibility of magnetic stent hyperthermia for the treatment of cardiovascular restenosis.
复制标题
磁性支架热疗治疗心血管再狭窄可行性的体外研究
DOI：
10.3892/etm.2013.1177
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发表时间：
2013-08
影响因子：
2.7
通讯作者：
Zhao LY
中科院分区：
文献类型：
--
作者：
Li L;Wang R;Shi HH;Xie L;Li JD;Kong WC;Tang JT;Ke DN;Zhao LY
热疗因其高效、安全、副作用少而受到广泛关注。在这项研究中，我们研究了是否有可能利用靶向热或支架内热治疗经皮冠状动脉腔内成形术（PTCA）后，通过磁支架热疗（MSH）治疗再狭窄。采用316L不锈钢支架和兔血管平滑肌细胞（VSMCs）为实验材料，研究交变磁场（AMF）作用下支架的感应加热特性，以及MSH对VSMCs增殖、凋亡、细胞周期和增殖细胞核抗原（PCNA）表达的影响。结果表明，在300 kHz AMF暴露下，316 L不锈钢冠状动脉支架具有理想的感应加热特性。加热性能被证明是由AMF的场强，以及支架轴的方向的影响。MSH对VSMCs的增殖和凋亡有明显的促进作用，且这种作用具有温度依赖性。43°C的温和温度对VSMC的生长影响可忽略不计，而高于47°C的MSH处理有效地抑制VSMC增殖并诱导凋亡。此外，47°C处理对VSMC迁移表现出显著和长期的抑制作用。结果表明，MSH有可能作为一种预防和治疗再狭窄的替代方法应用于临床。
Thermal treatment or hyperthermia has received considerable attention in recent years due to its high efficiency, safety and relatively few side-effects. In this study, we investigated whether it was possible to utilize targeted thermal or instent thermal treatments for the treatment of restenosis following percutaneous transluminal coronary angioplasty (PTCA) through magnetic stent hyperthermia (MSH). A 316L stainless steel stent and rabbit vascular smooth muscle cells (VSMCs) were used in the present study, in which the inductive heating characteristics of the stent under alternative magnetic field (AMF) exposure, as well as the effect of MSH on the proliferation, apoptosis, cell cycle and proliferating cell nuclear antigen (PCNA) expression of the rabbit VSMCs, were evaluated. The results demonstrated that 316L stainless steel coronary stents possess ideal inductive heating characteristics under 300 kHz AMF exposure. The heating properties were shown to be affected by the field intensity of the AMF, as well as the orientation the stent axis. MSH had a significant effect on the proliferation and apoptosis of VSMCs, and the effect was temperature-dependent. While a mild temperature of 43°C demonstrated negligible effects on the growth of VSMCs, MSH treatment above 47°C effectively inhibited the VSMC proliferation and induced apoptosis. Furthermore, a 47°C treatment exhibited a significant and long-term inhibitory effect on VSMC migration. The results strongly suggested that MSH may be potentially applied in the clinic as an alternative approach for the prevention and treatment of restenosis.
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DOI：
10.4028/www.scientific.net/kem.288-289.579
发表时间：
2005-01-01
期刊：
ASBM6: ADVANCED BIOMATERIALS VI
影响因子：
--
作者：
Li, CJ;Zheng, YF;Zhao, LC
通讯作者：
Zhao, LC
影响因子：
2.1
作者：
Oya, Junji;Shoji, Hodaka;Sato, Tadakuni
通讯作者：
Sato, Tadakuni
影响因子：
14
作者：
Wei, Yu;Ji, Ying;Ji, Jian
通讯作者：
Ji, Jian
影响因子：
14
作者：
Riegler, Johannes;Liew, Aaron;Lythgoe, Mark F.
通讯作者：
Lythgoe, Mark F.
影响因子：
5
作者：
Orihara, K;Biro, S;Tei, C
通讯作者：
Tei, C