Properties and application of a multichannel integrated circuit for low-artifact, patterned electrical stimulation of neural tissue.
Properties and application of a multichannel integrated circuit for low-artifact, patterned electrical stimulation of neural tissue.
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DOI：
10.1088/1741-2560/9/6/066005
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发表时间：
2012-12
影响因子：
4
通讯作者：
Dąbrowski W
中科院分区：
文献类型：
--
作者：
Hottowy P;Skoczeń A;Gunning DE;Kachiguine S;Mathieson K;Sher A;Wiącek P;Litke AM;Dąbrowski W
现代多电极阵列（MEA）系统可以记录来自数千个电极的神经元活动，但它们提供电刺激的时空模式的能力非常有限。此外，刺激相关的伪影显著限制了记录神经元对刺激的反应的能力。为了解决这些问题，我们设计了一个多通道集成电路的图案MEA为基础的电刺激，并评估其性能与离体小鼠和大鼠视网膜的实验。刺激芯片包括64个独立的刺激通道。每个通道都包括一个内部数模转换器，可配置为电流或电压源。刺激波形的形状由实时数据流针对每个通道独立地定义。此外，每个通道都配备有用于减少刺激伪影的电路。使用高密度MEA刺激/记录系统，我们有效地刺激了单个视网膜神经节细胞（RGC），并记录了神经元的反应，即使在刺激电极上也具有最小的失真。我们独立地刺激了大鼠视网膜中的RGC群体，并且使用电刺激脉冲的复杂时空模式，我们高保真地复制了这些细胞的子集的视觉诱发的尖峰活动。与目前最先进的MEA系统相比，Stimchip能够用更复杂的电脉冲序列刺激神经元细胞，并显着减少伪影。这为研究神经元对电刺激的反应开辟了新的可能性，无论是在神经科学研究的背景下，还是在神经假体设备的开发中。
Modern multielectrode array (MEA) systems can record the neuronal activity from thousands of electrodes, but their ability to provide spatio-temporal patterns of electrical stimulation is very limited. Furthermore, the stimulus-related artifacts significantly limit the ability to record the neuronal responses to the stimulation. To address these issues, we designed a multichannel integrated circuit for patterned MEA-based electrical stimulation and evaluated its performance in experiments with isolated mouse and rat retina. The Stimchip includes 64 independent stimulation channels. Each channel comprises an internal digital-to-analog converter that can be configured as a current or voltage source. The shape of the stimulation waveform is defined independently for each channel by the real-time data stream. In addition, each channel is equipped with circuitry for reduction of the stimulus artifact. Using a high-density MEA stimulation/recording system, we effectively stimulated individual retinal ganglion cells (RGCs) and recorded the neuronal responses with minimal distortion, even on the stimulating electrodes. We independently stimulated a population of RGCs in rat retina and, using a complex spatio-temporal pattern of electrical stimulation pulses, we replicated visually-evoked spiking activity of a subset of these cells with high fidelity. Compared with current state-of-the-art MEA systems, the Stimchip is able to stimulate neuronal cells with much more complex sequences of electrical pulses and with significantly reduced artifacts. This opens up new possibilities for studies of neuronal responses to electrical stimulation, both in the context of neuroscience research and in the development of neuroprosthetic devices.
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影响因子：
13.9
作者：
Fenno L;Yizhar O;Deisseroth K
通讯作者：
Deisseroth K
影响因子：
12.6
作者：
Heer, F.;Hafizovic, S.;Hierlemann, A.
通讯作者：
Hierlemann, A.
DOI：
10.1007/s10470-007-9125-x
发表时间：
2008-06-01
影响因子：
1.4
作者：
Hottowy, Pawel;Dabrowski, Wtadystaw;Wiacek, Piotr
通讯作者：
Wiacek, Piotr
影响因子：
2.5
作者：
Csicsvari, J;Henze, DA;Buzsáki, G
通讯作者：
Buzsáki, G
影响因子：
25
作者：
Fujisawa, Shigeyoshi;Amarasingham, Asohan;Buzsaki, Gyoergy
通讯作者：
Buzsaki, Gyoergy