Functional skeletal muscle formation with a biologic scaffold.
Functional skeletal muscle formation with a biologic scaffold.
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DOI：
10.1016/j.biomaterials.2010.06.039
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发表时间：
2010-10
期刊：
影响因子：
14
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
--
作者：
Valentin, Jolene E.;Turner, Neill J.;Gilbert, Thomas W.;Badylak, Stephen F.
在临床前研究和人类临床应用中，由细胞外基质（ECM）组成的生物支架已被用于增强或替换受损或缺失的肌肉肌腱组织。然而，大多数研究都集中在形态学终点，很少有研究评估新形成组织的原位功能，特别是新的骨骼肌组织。本研究的目的是确定在啮齿动物腹壁模型中使用四种供试品之一重建的骨骼肌组织的原位强直收缩反应和组织形态学特征：1）猪小肠粘膜下层（SIS）-ECM; 2）马来酰亚胺交联的猪SIS-ECM; 3）自体组织;或4）聚丙烯补片。手术后6个月，重塑的SIS-ECM显示出几乎完全被骨骼肌岛和片替代，其产生与天然组织相似的最大收缩力，但具有更大的抗疲劳性。自体组织移植物由胶原结缔组织、脂肪组织的混合物代替，与SIS-ECM相比具有更少的骨骼肌岛，并且与天然肌肉具有相似的抗疲劳性。碳化二亚胺交联的SIS-ECM和聚丙烯补片的特征在于慢性炎症反应，并且产生很少或没有可测量的强直力。这项研究的结果表明，非交联异种SIS支架和自体组织与功能性骨骼肌的恢复有关，其组织形态学特征类似于天然肌肉。
Biologic scaffolds composed of extracellular matrix (ECM) have been used to reinforce or replace damaged or missing musculotendinous tissues in both preclinical studies and in human clinical applications. However, most studies have focused upon morphologic endpoints and few studies have assessed the in-situ functionality of newly formed tissue; especially new skeletal muscle tissue. The objective of the present study was to determine both the in-situ tetanic contractile response and histomorphologic characteristics of skeletal muscle tissue reconstructed using one of four test articles in a rodent abdominal wall model: 1) porcine small intestinal submucosa (SIS)-ECM; 2) carbodiimide-crosslinked porcine SIS-ECM; 3) autologous tissue; or 4) polypropylene mesh. Six months after surgery, the remodeled SIS-ECM showed almost complete replacement by islands and sheets of skeletal muscle, which generated a similar maximal contractile force to native tissue but with greater resistance to fatigue. The autologous tissue graft was replaced by a mixture of collagenous connective tissue, adipose tissue with fewer islands of skeletal muscle compared to SIS-ECM and a similar fatigue resistance to native muscle. Carbodiimide-crosslinked SIS-ECM and polypropylene mesh were characterized by a chronic inflammatory response and produced little or no measureable tetanic force. The findings of this study show that non-crosslinked xenogeneic SIS scaffolds and autologous tissue are associated with the restoration of functional skeletal muscle with histomorphologic characteristics that resemble native muscle.
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DOI：
10.1002/jbm.b.31234
发表时间：
2009-05-01
影响因子：
3.4
作者：
Bellon, J. M.;Rodriguez, M.;Bujan, J.
通讯作者：
Bujan, J.
影响因子：
3.6
作者：
Borschel, GH;Dennis, RG;Kuzon, WM
通讯作者：
Kuzon, WM
影响因子：
2.2
作者：
Badylak, S;Kokini, M;Whitson, B
通讯作者：
Whitson, B
影响因子：
2.9
作者：
Druecke, D;Lamme, EN;Steinstraesser, L
通讯作者：
Steinstraesser, L
影响因子：
4.8
作者：
Ehrhardt, J;Morgan, J
通讯作者：
Morgan, J