Outgrowth of BT-474 human breast cancer cells in immune-deficient mice: a new in vivo model for hormone-dependent breast cancer.
Outgrowth of BT-474 human breast cancer cells in immune-deficient mice: a new in vivo model for hormone-dependent breast cancer.
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免疫缺陷小鼠中BT-474人类乳腺癌细胞的生长：一种新的体内体内模型，用于激素依赖性乳腺癌。
DOI：
10.1038/bjc.1995.271
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发表时间：
1995-07
影响因子：
8.8
通讯作者：
Smith, H. S.
中科院分区：
文献类型：
--
作者：
Van Slooten, H.-J.;Bonsing, B. A.;Hiller, A. J.;Colbern, G. T.;Van Dierendonck, J. H.;Cornelisse, C. J.;Smith, H. S.
在免疫缺陷小鼠中测试基底膜基质Matrigel和两种人乳腺癌细胞系BT-474和SK-BR-3的共接种的效果。两种细胞系都强烈过表达c-ErbB-2蛋白，而据报道只有BT-474是雌激素受体阳性的。共接种Matrigel和BT-474细胞而非Matrigel和SK-BR-3细胞导致bg-nu-xid小鼠中的肿瘤形成。雌激素的补充大大提高了致瘤性，但似乎并不是一个绝对的要求。在体内，BT-474细胞作为低分化腺癌生长，接种到颈部区域后倍增时间为9.4 +/- 1.1天。高增殖活性似乎被相对高的细胞损失率所补偿，因为BT-474肿瘤含有许多具有典型的凋亡细胞死亡形态的细胞。已知参与诱导细胞凋亡的野生型p53在肿瘤中不存在，而已知抑制细胞凋亡的Bcl-2以中等水平表达。BT-474肿瘤倾向于转移到局部淋巴结，并且能够在肺中形成微转移病灶。DNA倍性的流式细胞术分析表明，与细胞系相比，肿瘤没有变化。免疫组织化学和流式细胞术检测的一些激素和生长因子受体，转录因子，细胞粘附分子和蛋白质参与增殖和细胞死亡证明没有重大变化的倍性和肿瘤的表型相比，细胞系。细胞表面分子c-ErbB-2和episialin的高表达使其成为免疫治疗研究的潜在有用模型。
The effect of co-inoculation of basement membrane matrix, Matrigel and two human breast cancer cell lines, BT-474 and SK-BR-3, was tested in immune-deficient mice. Both cell lines strongly overexpress c-ErbB-2 protein, whereas only BT-474 is reported to be oestrogen receptor positive. Co-inoculation of Matrigel and BT-474 cells but not of Matrigel and SK-BR-3 cells resulted in tumour formation in bg-nu-xid mice. Oestrogen supplementation greatly enhanced tumorigenicity, but did not seem to be an absolute requirement. In vivo, BT-474 cells grow as a poorly differentiated adenocarcinoma with a doubling time of 9.4 +/- 1.1 days after inoculation into the neck region. A high proliferative activity appears to be compensated by a relatively high rate of cell loss, as BT-474 tumours contain many cells with the typical morphology of apoptotic cell death. Wild-type p53, known to participate in the induction of apoptosis, is absent from the tumours, whereas Bcl-2, known to inhibit apoptosis, is expressed at intermediate levels. BT-474 tumours tend to metastasise to the regional lymph nodes and are capable of forming micrometastatic lesions in the lung. Flow cytometrical analysis of DNA ploidy demonstrated no change in tumours compared with the cell line. Immunohistochemical and flow cytometrical detection of a number of hormone and growth factor receptors, transcription factors, cell adhesion molecules and proteins involved in proliferation and cell death demonstrated no major changes in ploidy and phenotype of tumours compared with the cell line. High expression of the cell-surface molecules c-ErbB-2 and episialin make it a potentially useful model for research in immune therapy.
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DOI：
10.1073/pnas.89.12.5645
发表时间：
1992-06-15
影响因子：
11.1
作者：
FU, XY;GUADAGNI, F;HOFFMAN, RM
通讯作者：
HOFFMAN, RM
影响因子：
9.6
作者：
HORGAN, K;JONES, DL;MANSEL, RE
通讯作者：
MANSEL, RE
影响因子：
6.4
作者：
ELLIOTT, BE;TAM, SP;CHEN, ZQ
通讯作者：
CHEN, ZQ
DOI：
10.1073/pnas.88.20.9345
发表时间：
1991-10-01
影响因子：
11.1
作者：
FU, XY;BESTERMAN, JM;HOFFMAN, RM
通讯作者：
HOFFMAN, RM
影响因子：
4
作者：
EVAN, GI;LITTLEWOOD, TD
通讯作者：
LITTLEWOOD, TD