12 Novel clonal groups of Leptospira infecting humans in multiple contrasting epidemiological contexts in Sri Lanka.
12 Novel clonal groups of Leptospira infecting humans in multiple contrasting epidemiological contexts in Sri Lanka.
复制标题
12在斯里兰卡多种截然不同的流行病学背景下感染人类的钩端螺旋体的新克隆群。
DOI：
10.1371/journal.pntd.0009272
复制
发表时间：
2021-03
影响因子：
3.8
通讯作者：
M Vinetz J
中科院分区：
文献类型：
--
作者：
Jayasundara D;Senavirathna I;Warnasekara J;Gamage C;Siribaddana S;Kularatne SAM;Matthias M;Mariet JF;Picardeau M;Agampodi S;M Vinetz J
钩端螺旋体病是一种普遍存在的人畜共患疾病，由于可能归因于钩端螺旋体多样性的多种临床表现和表现，因此是一项重大的临床挑战，对钩端螺旋体病的了解是研究这一新兴全球疾病威胁的流行病学的关键。斯里兰卡是钩端螺旋体病的热点地区，具有高度的地方性和年度流行性。我们在斯里兰卡进行了一项钩端螺旋体多样性的前瞻性研究，涵盖了气候带，地理和临床严重程度的全部范围。在两年半的时间里，从斯里兰卡25个区中的15个区的1 192名患者中采集了样本进行钩端螺旋体培养。25个分离株分别属于4种致病性钩端螺旋体：L.问号湖博格彼得森湖weilii和L. kirschneri。在分离株中鉴定出至少6个血清群：Autumnalis（6）、Pyrogenes（4）、黄疸出血群（2）、Celledoni（1）、流感伤寒群（2）和Bataviae（1）。7株分离株使用可用的抗血清板未发生凝集，表明存在新的血清群。使用Illumina平台对分离株进行测序。这些数据增加了25个新的核心基因组序列类型，并聚类在15个克隆组中，包括12个新的克隆组。L. borgpetersenii仅分布于干燥带，L.只有在潮湿的地方才有可能。急性肾损伤和心血管受累仅见于L。问号线虫感染血小板减少和肝功能损害均见于L。interrogans和L.伯氏菌感染。培养分离鉴定感染性钩端螺旋体的灵敏度不足，强调了对钩端螺旋体的非培养型分型方法的需要。世界范围内致病性钩端螺旋体物种存在巨大的多样性，由于对流行国家钩端螺旋体物种的分离和鉴定有限，我们对目前流行的物种的了解不足。这项前瞻性研究揭示了广泛的病原体多样性，导致人类钩端螺旋体病在斯里兰卡，代表四个物种，超过六个血清群，和15个克隆组。此外，观察到的不同地理和气候带分布和临床表现强调了前瞻性研究的必要性，以扩大分子流行病学方法来对抗钩端螺旋体病。
Leptospirosis is a ubiquitous zoonotic disease and a major clinical challenge owing to the multitude of clinical presentations and manifestations that are possibly attributable to the diversity of Leptospira, the understanding of which is key to study the epidemiology of this emerging global disease threat. Sri Lanka is a hotspot for leptospirosis with high levels of endemicity as well as annual epidemics. We carried out a prospective study of Leptospira diversity in Sri Lanka, covering the full range of climatic zones, geography, and clinical severity. Samples were collected for leptospiral culture from 1,192 patients from 15 of 25 districts in Sri Lanka over two and half years. Twenty-five isolates belonging to four pathogenic Leptospira species were identified: L. interrogans, L. borgpetersenii, L. weilii, and L. kirschneri. At least six serogroups were identified among the isolates: Autumnalis (6), Pyrogenes (4), Icterohaemorrhagiae (2), Celledoni (1), Grippotyphosa (2) and Bataviae (1). Seven isolates did not agglutinate using available antisera panels, suggesting new serogroups. Isolates were sequenced using an Illumina platform. These data add 25 new core genome sequence types and were clustered in 15 clonal groups, including 12 new clonal groups. L. borgpetersenii was found only in the dry zone and L. weilii only in the wet zone. Acute kidney injury and cardiovascular involvement were seen only with L. interrogans infections. Thrombocytopenia and liver impairment were seen in both L. interrogans and L. borgpetersenii infections. The inadequate sensitivity of culture isolation to identify infecting Leptospira species underscores the need for culture-independent typing methods for Leptospira. There is a huge diversity in pathogenic Leptospira species worldwide, and our knowledge of the currently circulating species is deficient owing to limited isolation and identification of Leptospira species from endemic countries. This prospective study reveals the wide pathogen diversity that causes human leptospirosis in Sri Lanka, representing four species, more than six serogroups, and fifteen clonal groups. Further, the different geographic and climatic zone distributions and clinical manifestations observed underscores the need for prospective studies to expand the molecular epidemiological approaches to combat leptospirosis.
登录
查看更多内容
影响因子：
4.3
作者：
Gamage, C. D.;Koizumi, N.;Tamashiro, H.
通讯作者：
Tamashiro, H.
DOI：
10.1016/j.diagmicrobio.2017.01.014
发表时间：
2017-05-01
影响因子：
2.9
作者：
Girault, Dominique;Soupe-Gilbert, Marie-Estelle;Goarant, Cyrille
通讯作者：
Goarant, Cyrille
影响因子：
11.8
作者：
Gouvela, Edilane L.;Metcalfe, John;Ko, Albert I.
通讯作者：
Ko, Albert I.
影响因子：
3.7
作者：
Herath, Nalaka;Uluwattage, Wimalasiri;Agampodi, Suneth
通讯作者：
Agampodi, Suneth
影响因子：
3.4
作者：
Llanes A;Restrepo CM;Riesgo-Ferreiro P;Rajeev S
通讯作者：
Rajeev S