Effect of Glycosylation on Self-Assembly of Lipid A Lipopolysaccharides in Aqueous Solutions.
Effect of Glycosylation on Self-Assembly of Lipid A Lipopolysaccharides in Aqueous Solutions.
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DOI：
10.1021/acs.langmuir.3c00828
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发表时间：
2023-06-20
期刊：
影响因子：
3.9
通讯作者：
Hamley, Ian W. W.
中科院分区：
文献类型：
--
作者：
Castelletto, Valeria;Seitsonen, Jani;Hamley, Ian W. W.
由细菌产生的基于脂质A的脂多糖（LPS）由于其在刺激免疫应答中的生物活性而受到关注，更简单的合成组分或类似物也是如此。在这里，在水中的两个单分散的脂质A衍生物的基础上简化的细菌LPS结构的自组装进行检查和比较，使用小角X-射线散射和低温透射电子显微镜的本地大肠杆菌LPS。通过荧光探针实验获得了临界聚集浓度，并利用圆二色光谱对其构象进行了探测。急诊大肠杆菌LPS形成蠕虫状胶束，而合成的类似物带有六个脂质链和四个或两个糖头基团（Kdo 2-lipid A和monophosphoryl lipid A）自组装成纳米片或囊泡，分别。这些意见是合理的，考虑到表面活性剂的包装参数。
Lipopolysaccharides (LPSs) based on lipid A produced by bacteria are of interest due to their bioactivity in stimulating immune responses, as are simpler synthetic components or analogues. Here, the self-assembly in water of two monodisperse lipid A derivatives based on simplified bacterial LPS structures is examined and compared to that of a native Escherichia coli LPS using small-angle X-ray scattering and cryogenic transmission electron microscopy. The critical aggregation concentration is obtained from fluorescence probe experiments, and conformation is probed using circular dichroism spectroscopy. The E. coli LPS is found to form wormlike micelles, whereas the synthetic analogues bearing six lipid chains and with four or two saccharide head groups (Kdo2-lipid A and monophosphoryl lipid A) self-assemble into nanosheets or vesicles, respectively. These observations are rationalized by considering the surfactant packing parameter.
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影响因子：
15
作者：
KALYANASUNDARAM, K;THOMAS, JK
通讯作者：
THOMAS, JK
影响因子：
3.9
作者：
Andreev, Konstantin;Martynowycz, Michael W.;Ivankin, Andrey;Huang, Mia L.;Kuzmenko, Ivan;Meron, Mati;Lin, Binhua;Kirshenbaum, Kent;Gidalevitz, David
通讯作者：
Gidalevitz, David
影响因子：
56.9
作者：
Mata-Haro, Veronica;Cekic, Caglar;Mitchell, Thomas C.
通讯作者：
Mitchell, Thomas C.
影响因子：
3.1
作者：
Martin, M;Michalek, SM;Katz, J
通讯作者：
Katz, J
DOI：
10.1039/f29767201525
发表时间：
1976-01-01
期刊：
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS II
影响因子：
--
作者：
ISRAELACHVILI, JN;MITCHELL, DJ;NINHAM, BW
通讯作者：
NINHAM, BW