An SSR-based genetic map of pepper (Capsicum annuum L.) serves as an anchor for the alignment of major pepper maps.
An SSR-based genetic map of pepper (Capsicum annuum L.) serves as an anchor for the alignment of major pepper maps.
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DOI：
10.1270/jsbbs.62.93
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发表时间：
2012-03
期刊：
影响因子：
2.4
通讯作者：
Kubo N
中科院分区：
文献类型：
--
作者：
Mimura Y;Inoue T;Minamiyama Y;Kubo N
在辣椒（Capsicum spp.）中，人工栽培的C. annuum是最重要的商业品种，但缺乏基于序列特异性PCR标记的与单倍体染色体数目一致的种内连锁图谱。我们利用两种辣椒基因型的双单倍体（DH）群体构建了辣椒连锁图谱，这两种基因型是钟型栽培品种“加利福尼亚Wonder”和马来西亚小果栽培品种“LS2341 （JP187992）”，该品种被用作抗青黄病（Ralstonia solanacearum）的来源。我们构建了一套253个标记（151个SSRs， 90个aflp， 10个CAPSs和2个序列标记位点），全长1,336 cM。这是第一个基于ssr的图谱，包含12个连锁群，对应于种内杂交的单倍体染色体数目。由于该图谱包含大量基于pcr的锚点标记，便于与其他辣椒遗传图谱进行比较。因此，该图谱和新开发的标记将为栽培黄杨的选育提供参考。
Of the Capsicum peppers (Capsicum spp.), cultivated C. annuum is the most commercially important, but has lacked an intraspecific linkage map based on sequence-specific PCR markers in accord with haploid chromosome numbers. We constructed a linkage map of pepper using a doubled haploid (DH) population derived from a cross between two C. annuum genotypes, a bell-type cultivar ‘California Wonder’ and a Malaysian small-fruited cultivar ‘LS2341 (JP187992)’, which is used as a source of resistance to bacterial wilt (Ralstonia solanacearum). A set of 253 markers (151 SSRs, 90 AFLPs, 10 CAPSs and 2 sequence-tagged sites) was on the map which we constructed, spanning 1,336 cM. This is the first SSR-based map to consist of 12 linkage groups, corresponding to the haploid chromosome number in an intraspecific cross of C. annuum. As this map has a lot of PCR-based anchor markers, it is easy to compare it to other pepper genetic maps. Therefore, this map and the newly developed markers will be useful for cultivated C. annuum breeding.
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影响因子：
1.9
作者：
Huang, SW;Zhang, BX;Guo, JZ
通讯作者：
Guo, JZ
影响因子：
1.9
作者：
LANTERI, S;PICKERSGILL, B
通讯作者：
PICKERSGILL, B
影响因子：
5.4
作者：
Ben Chaim, A;Paran, I;Peleman, J
通讯作者：
Peleman, J
DOI：
10.2503/jjshs1.79.56
发表时间：
2010-01-01
影响因子：
--
作者：
Mimura, Yutaka;Minamiyama, Yasuhiro;Hirai, Masashi
通讯作者：
Hirai, Masashi
影响因子：
3.1
作者：
Nagy, Istvan;Stagel, Anik;Ganal, Martin
通讯作者：
Ganal, Martin